| Budget Amount *help |
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2013: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Outline of Final Research Achievements |
Muscle stem cells express calcitonin receptor, but its physiological role was unclear. We generated Calcr conditional KO mice and analyzed muscle stem cell and muscle regeneration ability. Intriguingly, the mice exhibited the decreased number of MuSC and impaired regenerative potential. We also generated Hey1-HeyL conditional KO mice, and found the ectopic expression of myogenic genes, which suggests the cell-autonomous role of Hey1-HeyL in muscle stem cells. Using microarray analyses, Nrf2 was identified as the down stream target of Notch and Hey1-HeyL. Nrf2 target genes were downregulated in Nrf2-KO mice, but the KO mice showed no abnormality in muscle stem cells and acute muscle injury model.
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