The roles of dormant genes in muscle stem cells
Project/Area Number |
25702044
|
Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Partial Multi-year Fund |
Research Field |
Applied health science
|
Research Institution | Osaka University |
Principal Investigator |
Fukada So-ichiro 大阪大学, 薬学研究科(研究院), 准教授 (20432445)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2013: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
|
Keywords | 骨格筋 / 筋衛星細胞 / 幹細胞 / カルシトニン / Notch / 維持機構 |
Outline of Final Research Achievements |
Muscle stem cells express calcitonin receptor, but its physiological role was unclear. We generated Calcr conditional KO mice and analyzed muscle stem cell and muscle regeneration ability. Intriguingly, the mice exhibited the decreased number of MuSC and impaired regenerative potential. We also generated Hey1-HeyL conditional KO mice, and found the ectopic expression of myogenic genes, which suggests the cell-autonomous role of Hey1-HeyL in muscle stem cells. Using microarray analyses, Nrf2 was identified as the down stream target of Notch and Hey1-HeyL. Nrf2 target genes were downregulated in Nrf2-KO mice, but the KO mice showed no abnormality in muscle stem cells and acute muscle injury model.
|
Report
(4 results)
Research Products
(26 results)
-
[Journal Article] Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche2015
Author(s)
Yamaguchi M, Watanabe Y, Ohtani T, Uezumi A, Mikami N, Nakamura M, Sato T, Ikawa M, Hoshino M, Tsuchida K, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H, Fukada S
-
Journal Title
Cell Rep
Volume: 13(2)
Issue: 2
Pages: 302-314
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Evidence of Notch-Hesr-Nrf2 Axis in Muscle Stem Cells, but Absence of Nrf2 Has No Effect on Their Quiescent and Undifferentiated Stat2015
Author(s)
Yamaguchi M, Murakami S, Yoneda T, Nakamura M, Zhang L, Uezumi A, Fukuda S, Kokubo H, Tsujikawa K, Fukada S
-
Journal Title
PLoS One
Volume: 10(9)
Issue: 9
Pages: 138517-138531
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
[Journal Article] Doublecortin marks a new population of transiently amplifying muscle progenitor cells and is required for myofiber maturation during skeletal muscle regeneration2015
Author(s)
Ogawa R, Ma Y, Yamaguchi M, Ito T, Watanabe Y, Ohtani T, Murakami S, Uchida S, De Gaspari P, Uezumi A, Nakamura M, Miyagoe-Suzuki Y, Tsujikawa K, Hashimoto N, Braun T, Tanaka T, Takeda S, Yamamoto H, Fukada S
-
Journal Title
Development
Volume: 142
Issue: 1
Pages: 51-61
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] Doublecortin marks a new population of transiently amplifying muscle progenitor cells and is required for myofiber maturation during skeletal muscle regeneration.2015
Author(s)
Ogawa R, Ma Y, Yamaguchi M, Ito T, Watanabe Y, Ohtani T, Murakami S, Uchida S, De Gaspari P, Uezumi A, Nakamura M, Miyagoe-Suzuki Y, Tsujikawa K, Hashimoto N, Braun T, Tanaka T, Takeda S, Yamamoto H, Fukada S.
-
Journal Title
Development
Volume: 142
Pages: 51-61
Related Report
Peer Reviewed / Acknowledgement Compliant
-
[Journal Article] Identification and characterization of PDGFRalpha(+) mesenchymal progenitors in human skeletal muscle2014
Author(s)
Uezumi, A. Fukada, S. Yamamoto, N. Ikemoto-Uezumi, M. Nakatani, M. Morita, M. Yamaguchi, A. Yamada, H. Nishino, I. Hamada, Y. Tsuchida, K.
-
Journal Title
Cell Death Dis
Volume: In press
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-