| Budget Amount *help |
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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| Outline of Final Research Achievements |
In this study, I have conducted neurotoxins and proteases from venomous mammals, including shrews and solenodons. The short-tailed shrew (Blarina brevicauda) produce a lethal protein venom in saliva. After removing proteins by ultracentrifugation, the smaller molecular weight fraction showed significant paralytic activity against mealworms and mice (icv injection). Based on this activity, two peptide neurotoxins with molecular weights of 5.1 and 5.7 kDa were purified, and their primary amino acid sequences were established. As for Cuban solenodon (Solenodon cubanus), ecological survey was conducted in a national park in Cuba. Several individuals were successfully taken alive, and their saliva was collected. The partial amino acid sequences and activity of one of the major proteases in solenodon saliva was determined.
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