Development of versatile N-oxide-based fluorescent probes to study pathological dysfunction of iron metabolism
| Project/Area Number |
25702050
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Gifu Pharmaceutical University |
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Principal Investigator |
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| Research Collaborator |
NIWA Masato
TSUBOI Hitomi
KADOTA Satoki
IKESHITA Yukie
MIKI Ayaji
KATABE Tomoyo
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
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| Keywords | 鉄 / イメージング / 蛍光プローブ / 鉄イオン / 鉄代謝 / 蛍光イメージング |
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| Outline of Final Research Achievements |
We have developed a series of fluorescent probes for Fe(II) ion, which is an important chemical species in the iron metabolism, to study biological and pathological roles of iron ion in living cells. The design concept of Fe(II) fluorescent probe is based on N-oxide chemistry, where Fe(II) ion selectively reduces N-oxide by deoxygenation. In this research, we have succeeded in development of a color variation of the probes ranging from blue to deep-red by utilizing N-oxide chemistry as a selective fluorogenic molecular switch. We have also developed a membrane-anchoring fluorescent probe to visualize release of Fe(II) ion during endocytotic uptake of iron induced by transferrin/transferrin receptor interaction.
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Report
(5 results)
Research Products
(55 results)
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[Journal Article] Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis.2016
Author(s)
Wang Y, Okazaki Y, Shi L, Kohda H, Tanaka M, Taki K, Nishioka T, Hirayama T, Nagasawa H, Yamashita Y, Toyokuni S.
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Journal Title
Cancer Sci
Volume: 107(3)
Issue: 3
Pages: 250-257
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Nordihydroguaiaretic acid disrupts the antioxidant ability of Helicobacter pylori through the repression of SodB activity in vitro.2015
Author(s)
Tsugawa, H., Mori, H., Matsuzaki, J., Masaoka, T., Hirayama, T., Nagasawa, H., Sakakibara, Y., Suematsu, M., Suzuki, H.
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Journal Title
Biomed. Res. Int.
Volume: 2015
Pages: 734548-734548
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Ovarian endometriosis-associated stromal cells reveal persistently high affinity for iron2015
Author(s)
M. Mori, F. Ito, L. W. Shi, C. Ishida, Y. Hattori, M. Niwa, T. Hirayama, H. Nagasawa, A. Iwase, F. Kikkawa, S. Toyokuni
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Journal Title
Redox Biology
Volume: 6
Pages: 578-586
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Catalytic ferrous ion in amniotic fluid as a predictive marker of human maternal-fetal disorders2015
Author(s)
Y. Hattori, T. Mukaide, L. Jiang, T. Kotani, H. Tsuda, Y. Mano, S. Sumigawa, T. Hirayama, H. Nagasawa, F. Kikkawa, S. Toyokuni
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Journal Title
Journal of Clinical Biochemistry and Nurition
Volume: 56
Pages: 57-63
Related Report
Peer Reviewed
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