| Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
|
|---|
| Outline of Final Research Achievements |
We have developed a series of fluorescent probes for Fe(II) ion, which is an important chemical species in the iron metabolism, to study biological and pathological roles of iron ion in living cells. The design concept of Fe(II) fluorescent probe is based on N-oxide chemistry, where Fe(II) ion selectively reduces N-oxide by deoxygenation. In this research, we have succeeded in development of a color variation of the probes ranging from blue to deep-red by utilizing N-oxide chemistry as a selective fluorogenic molecular switch. We have also developed a membrane-anchoring fluorescent probe to visualize release of Fe(II) ion during endocytotic uptake of iron induced by transferrin/transferrin receptor interaction.
|
