| Budget Amount *help |
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2016: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2014: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
We have generated transgenic mice that express, in astrocytes of the glial cell population, fluorescent proteins that are sensitive to changes in intracellular pH or calcium. Compared to methods using synthetic dyes, these proteins are specifically expressed in astrocytes. Therefore, using acute brain slice preparations, local changes in the fine processes of the cells can be readily identified. In addition, small cranial window was created and optical fiber was inserted to observe the intracellular ionic changes in vivo. These methods along with the methods to optical control intracellular ionic changes using ChR2 and ArchT, we would be able to understand how astrocytes function affect neuronal activity and animal behavior.
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