Single molecule detection of protein/DNA aptamer complex using giant biological nanpores
| Project/Area Number |
25708024
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
KAWANO Ryuji 東京農工大学, 工学(系)研究科(研究院), 准教授 (90401702)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2013: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
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| Keywords | 電気化学 / 一分子計測 / チャネル膜タンパク質 / 脂質二分子膜 / マイクロ加工 / ナノポア / アプタマー |
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| Outline of Final Research Achievements |
We have developed stable lipid bilayer using droplet contact method. This method allows us to measure current signals of larger-sized nanopore proteins with the portable system. Using this system, we are able to measure a serine protease and a toxin protein with DNA aptamer using the large nanopore at the single molecule level.
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Report
(3 results)
Research Products
(10 results)
