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Development of innovative technologies to visualize and control RNAs in living cells

Research Project

Project/Area Number 25708025
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Bio-related chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

Yoshimura Hideaki  東京大学, 理学(系)研究科(研究院), 助教 (90464205)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2013: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
KeywordsRNA / イメージング / 1分子観察 / テロメア
Outline of Final Research Achievements

I developed a probe to visualize single-molecule dynamics of telomere repeat-containing RNA (TERRA) in living cells to reveal mechanism of TERRA function in telomere maintenance. I performed single molecule imaging of TERRA and telomere in living U2OS cells using the present probe. Analysis of spatial distribution of TERRA and telomere revealed that TERRA intensively localize in a region 1-2 μm apart from a telomere. Next I performed simultaneous single molecule observation of TERRA, telomere, and hnRNPA1 which is a telomere-related protein. The results of this observation showed that TERRA and hnRNPA1 frequently colocalize to each other in the telomere-surrounding region. On the basis of these results, I propose a novel mechanistic model of TERRA, in which TERRA in the telomere-surrounding region traps hnRNPA1 and control the function of hnRNPA1 to maintain telomere length.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (33 results)

All 2016 2015 2014 2013

All Journal Article (7 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (26 results) (of which Int'l Joint Research: 5 results,  Invited: 6 results)

  • [Journal Article] Genetically Encoded Fluorescent Probe for Imaging Apoptosis in Vivo with Spontaneous GFP Complementation.2016

    • Author(s)
      Yusuke Nasu, Yoichi Asaoka, Misako Namae, Hiroshi Nishina, Hideaki Yoshimura and Takeaki Ozawa
    • Journal Title

      Anal Chem

      Volume: 88 Issue: 1 Pages: 838-844

    • DOI

      10.1021/acs.analchem.5b03367

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of red-shifted mutants derived from luciferase of Brazilian click beetle Pyrearinus termitilluminans2015

    • Author(s)
      Tomoki Nishiguchi, Toshimichi Yamada, Yusuke Nasu, Mashiho Ito, Hideaki Yoshimura, Takeaki Ozawa
    • Journal Title

      Journal of Biomedical Optics

      Volume: 20 Issue: 10 Pages: 101205-101205

    • DOI

      10.1117/1.jbo.20.10.101205

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RNAと生細胞内1分子イメージングの可能性2015

    • Author(s)
      吉村英哲
    • Journal Title

      Labcab

      Volume: 12 Pages: 19-20

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 生細胞内RNAイメージング2015

    • Author(s)
      吉村英哲、小澤岳昌
    • Journal Title

      細胞工学

      Volume: 34 Pages: 53-58

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Simultaneous time-lamination imaging of protein association using a split fluorescent timer protein2015

    • Author(s)
      A. Takamura, M. Hattori, H. Yoshimura and T. Ozawa
    • Journal Title

      Anal. Chem.

      Volume: 87 Issue: 6 Pages: 3366-3372

    • DOI

      10.1021/ac504583t

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 蛍光顕微鏡を用いた生細胞内1分子可視化解析法2015

    • Author(s)
      吉村英哲、小澤岳昌
    • Journal Title

      The Bulletin of the Society of Nano Science and Technology

      Volume: 13 Pages: 61-65

    • Related Report
      2014 Annual Research Report
  • [Journal Article] Methods of split-reporter reconstitution for the analysis of biomolecules.2014

    • Author(s)
      H. Yoshimura and T. Ozawa
    • Journal Title

      Chem. Record

      Volume: 14 Issue: 3 Pages: 492-501

    • DOI

      10.1002/tcr.201402001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Analysis of single molecule dynamic to reveal the functional mechanism of telomeric repeat-containing RNA2016

    • Author(s)
      YOSHIMURA, Hideaki; YAMADA, Toshimichi; OZAWA, Takeaki
    • Organizer
      第95日本化学会春季年会
    • Place of Presentation
      同志社大学京田辺キャンパス(京都府・京田辺市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] SINGLE-MOLECULE LIVE-CELL IMAGING OF A NON-CODING RNA2016

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Rintaro Shimada, Takeaki Ozawa
    • Organizer
      Focus on Microscopy 2016
    • Place of Presentation
      台北(台湾)
    • Year and Date
      2016-03-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of a genetically-encoded probe to visualize β-actin mRNA in living cells based on a reconstituted GFP and an RNA binding domain PUM-HD2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Takeaki Ozawa
    • Organizer
      Pacifichem2015
    • Place of Presentation
      Honolulu (U.S.)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A fluorescent probe for single-molecule live-cell imaging of telomeric-repeat containing RNA using fluorescent protein reconstitution and an RNA-binding domain PUM-HD2015

    • Author(s)
      Hideaki Yoshimura, Toshimchi Yamada, Rintaro Shimada, Takeaki Ozawa
    • Organizer
      Pacifichem2015
    • Place of Presentation
      Honolulu (U.S.)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Single molecule imaging of telomeric-repeat containing RNA in living cells2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Takeaki Ozawa
    • Organizer
      9th International Symposium on Nanomedicine
    • Place of Presentation
      三重大学(三重県・津市)
    • Year and Date
      2015-12-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Single molecule imaging of telomeric repeat containing RNA in living cells2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Hiroki Segawa, Takeaki ozawa
    • Organizer
      第53回生物物理学会
    • Place of Presentation
      金沢大学 角間キャンパス(石川県・金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] テロメアRNA生細胞内1分子動態の定量評価法2015

    • Author(s)
      吉村英哲、山田俊理、瀬川尋貴、小澤岳昌
    • Organizer
      分析化学会第64年会
    • Place of Presentation
      九州大学伊都キャンパス(福岡県・福岡市)
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Analysis of dynamics on a non-coding RNA in living cells using a single molecule tracking approach2015

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Hiroki Segawa, Takeaki Ozawa
    • Organizer
      JASIS RSC Tokyo international conference
    • Place of Presentation
      幕張メッセ(千葉県・千葉市)
    • Year and Date
      2015-09-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞内1分子動態分析に基づくテロメアRNAの作動機構解析法2015

    • Author(s)
      吉村英哲、山田俊理、瀬川尋貴、小澤岳昌
    • Organizer
      第75回分析化学討論会
    • Place of Presentation
      山梨大学(山梨県・甲府市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Single molecule imaging to reveal mechanisms of complex cellular systems2015

    • Author(s)
      Hideaki Yoshimura
    • Organizer
      NTU-SNU-UT Chemistry Symposium
    • Place of Presentation
      台北(台湾)
    • Year and Date
      2015-01-16
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Single molecule imaging of Akt on the plasma membrane in living cells2014

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      「細胞のメゾスケール構造機能」シンポジウム
    • Place of Presentation
      京都大学iCeMS本部棟(京都府京都市)
    • Year and Date
      2014-12-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Single molecule imaging in living cells to reveal the relationship between motions and functions of biological molecules2014

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      8th International Symposium on Nanomedicine
    • Place of Presentation
      愛媛大学(愛媛県松山市)
    • Year and Date
      2014-12-04 – 2014-12-06
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Signal transduction mechanism of Akt reveald by single molecule imaging of Akt and receptor molecules2014

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      第52回生物物理学会年会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 生細胞1分子動態分析によるシグナル伝達タンパク質Aktの機能発現機構解明2014

    • Author(s)
      吉村英哲・小澤岳昌
    • Organizer
      第65回分析化学会年会
    • Place of Presentation
      広島大学東広島キャンパス(広島県東広島市)
    • Year and Date
      2014-09-15 – 2014-09-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Analysis of single-molecule dynamics of signal transductino molecule Akt in living cells2014

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      RSC-TIC JAIMA conference 幕張
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2014-09-04 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] Signal transduction mechanism of Akt based on single molecule dynamics in livng cells2014

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      第37回 内藤コンファレンス
    • Place of Presentation
      ニセコヒルトンビレッジ(北海道ニセコ町)
    • Year and Date
      2014-07-15 – 2014-07-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 新規蛍光プローブ開発を通じたテロメアRNAの生細胞内1分子動態分析2014

    • Author(s)
      吉村英哲・小澤岳昌
    • Organizer
      第74回 分析化学討論会
    • Place of Presentation
      日本大学郡山キャンパス(福島県郡山市)
    • Year and Date
      2014-05-24 – 2014-05-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] A study of a molecular mechanism of intracellular signal transduction based on single-molecule imaging2014

    • Author(s)
      Hideaki Yoshimura, Takeaki Ozawa
    • Organizer
      The 2nd Japan-China Symposium on Nanomedicine
    • Place of Presentation
      広島大学霞キャンパス(広島県広島市)
    • Year and Date
      2014-05-16 – 2014-05-17
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Live Cell Imaging of Single-Molecule RNAs Using PUM-HD Based RNA Probes2014

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Asumi Inaguma, Takeaki Ozawa
    • Organizer
      Focus on Microscopy 2014 (FOM 2014)
    • Place of Presentation
      シドニー(オーストラリア)
    • Year and Date
      2014-04-13 – 2014-04-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] Fluorescence Imaging of Telomeric Repeat-Containing RNA in Living Cells2014

    • Author(s)
      山田俊理・吉村英哲・服部 満・小澤岳昌
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] Live-cell imaging of single β-actin mRNAs through development of a specific RNA labeling method2013

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Asumi Inaguma, Takeaki Ozawa
    • Organizer
      RSC-TIC2013 (JASISカンファレンス)
    • Place of Presentation
      幕張メッセ
    • Related Report
      2013 Annual Research Report
  • [Presentation] 緑色蛍光タンパク質を利用した内在性テロメアRNAの一分子イメージング法の開発2013

    • Author(s)
      山田 俊理・吉村 英哲・服部 満・小澤 岳昌
    • Organizer
      日本分析化学会第62年会
    • Place of Presentation
      近畿大学東大阪キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生細胞内における内在性テロメアRNA可視化法の開発2013

    • Author(s)
      山田俊理・吉村英哲・服部 満・小澤岳昌
    • Organizer
      第3回CSJ化学フェスタ2013
    • Place of Presentation
      タワーホール船堀
    • Related Report
      2013 Annual Research Report
  • [Presentation] Single molecule imaging of endogenous telomeric RNA in living cells2013

    • Author(s)
      山田俊理・吉村英哲・服部 満・小澤岳昌
    • Organizer
      第 51 回日本生物物理学会年会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Visualization of molecular motion in living cells using a single molecule imaging method2013

    • Author(s)
      Hideaki Yoshimura
    • Organizer
      錯体化学会第63回討論会
    • Place of Presentation
      琉球大学千原キャンパス
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Analysis of RNA dynamics in living cells based on single molecule imaging2013

    • Author(s)
      Hideaki Yoshimura, Toshimichi Yamada, Hiroki Segawa, Asumi Inaguma, Takeaki Ozawa
    • Organizer
      7th International symposium on nanomedicine (ISNM2013)
    • Place of Presentation
      九州工業大学
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2013-05-21   Modified: 2019-07-29  

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