| Budget Amount *help |
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
|
|---|
| Outline of Final Research Achievements |
In this project, I succeeded in development of various chemical methodologies for protein-protein interactions (PPIs), such as cytokine-receptor interaction, or antigen-antibody interaction. Moreover, I realized the identification of interaction site by using PPI-based chemical modification method on living cells, and real-time imaging of the receptors on living cells. Additionally, development of self-assembling nanoprobes for live imaging of protein activity have been achieved, and this is the first example of reversible turn-on nanoprobes inside the live cells. Finally, based on these fundamental analyses, I succeeded in development of analysis methods for phytohormone receptors which cause protein-protein interaction and regulate life cycle in planta.
|
