Evaluation of non-culturable waterborne virus removal in drinking water treatment processes and innovation of virus removal technology

• Project/Area Number: 25709044
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Civil and environmental engineering
• Research Institution: Hokkaido University
• Principal Investigator: SHIRASAKI NOBUTAKA 北海道大学, 工学(系)研究科(研究院), 助教 (60604692)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000); Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2014: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 水系感染症 / 水系感染症ウイルス / ウイルス様粒子 / 新規Immuno-PCR法 / 新規凝集剤 / アルミニウム多量体 / インライン凝集 / 膜ろ過処理 / アデノウイルス / コクサッキーウイルス / A型肝炎ウイルス / マウスノロウイルス / ポリオウイルス / アルミ二ウム系凝集剤 / アルミ二ウム種 / アルミ二ウム多量体 / 塩基度 / 凝集沈澱処理 / ノロウイルス / フロック化 / ケーキ層形成 / 膜ファウリング

Outline of Final Research Achievements

We were able to successfully evaluate norovirus removal performance as particles during membrane filtration processes by using recombinant norovirus virus-like particles and the immuno-PCR method developed in the present study. In addition, a novel aluminum-based coagulant for effective virus removal was also successfully developed in the present study. A combination of coagulation pretreatment with the novel coagulant and microfiltration can provide effective removal of waterborne viruses including adenovirus and norovirus (more than 4 log) over a broader pH range than is possible with commercially available aluminum-based coagulant.

Research Products

• [Journal Article] Investigation of enteric adenovirus and poliovirus removal by coagulation processes and suitability of bacteriophages MS2 and φX174 as surrogates for those viruses (2016)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Marubayashi, T. and Murai, K.
  • Journal Title: Science of the Total Environment Volume: 掲載決定 Pages: 29-39
  • DOI: 10.1016/j.scitotenv.2016.04.090
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of coagulant basicity on virus removal from water by polyferric chloride (2016)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y. and Marubayashi, T.
  • Journal Title: Journal of Water Supply: Research and Technology: AQUA Volume: - Issue: 4 Pages: 322-329
  • DOI: 10.2166/aqua.2016.104
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Characterization of recombinant norovirus virus-like particles and evaluation of their applicability to the investigation of norovirus removal performance in membrane filtration processes (2016)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y. and Ohno, K
  • Journal Title: Water Science and Technology: Water Supply Volume: - Issue: 3 Pages: 737-745
  • DOI: 10.2166/ws.2015.185
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of aluminum hydrolyte species on human enterovirus removal from water during the coagulation process (2016)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y. and Marubayashi, T.
  • Journal Title: Chemical Engineering Journal Volume: 284 Pages: 786-793
  • DOI: 10.1016/j.cej.2015.09.045
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Virus removal by an in-line coagulation-ceramic microfiltration process with high-basicity polyaluminum coagulation pretreatment (2014)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Urasaki, T., Kimura, M. and Ohno, K.
  • Journal Title: Water Science and Technology: Water Supply Volume: 14(3) Issue: 3 Pages: 429-437
  • DOI: 10.2166/ws.2013.218
  • Peer Reviewed / Open Access
• [Journal Article] Improved virus removal by high-basicity polyaluminum coagulants compared to commercially available aluminum-based coagulants (2014)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Oshiba, A., Marubayashi, T. and Sato, S.
  • Journal Title: Water Research Volume: 48 Pages: 375-386
  • DOI: 10.1016/j.watres.2013.09.052
  • Peer Reviewed
• [Journal Article] Investigating norovirus removal by microfiltration, ultrafiltration, and precoagulation-microfiltration processes using recombinant norovirus virus-like particles and real-time immuno-PCR (2013)
  • Author(s): Matsushita, T., Shirasaki, N., Tatsuki, Y. and Matsui, Y.
  • Journal Title: Water Research Volume: 47 Issue: 15 Pages: 5819-5827
  • DOI: 10.1016/j.watres.2013.07.004
  • Peer Reviewed
• [Journal Article] Adsorptive virus removal with super-powdered activated carbon (2013)
  • Author(s): Matsushita, T.
  • Journal Title: Separation and Purification Technology Volume: 107 Pages: 79-84
  • DOI: 10.1016/j.seppur.2013.01.017
  • Peer Reviewed
• [Presentation] 消毒耐性ウイルスの膜ろ過処理性評価および代替指標候補ウイルスとの処理性比較 (2016)
  • Author(s): 村井一真, 白崎伸隆, 松井佳彦, 松下拓
  • Organizer: 第50回日本水環境学会年会
  • Place of Presentation: アスティとくしま, 徳島県徳島市
  • Year and Date: 2016-03-16
• [Presentation] トウガラシ微斑ウイルスは水系感染症ウイルスの浄水処理性指標となるのか？：凝集沈澱・砂ろ過における処理性比較 (2016)
  • Author(s): 山下玲菜, 白崎伸隆, 松下拓, 松井佳彦
  • Organizer: 第50回日本水環境学会年会
  • Place of Presentation: アスティとくしま, 徳島県徳島市
  • Year and Date: 2016-03-16
• [Presentation] Contaminant Candidate Listに掲載された水系感染症ウイルスの凝集処理性評価 (2014)
  • Author(s): 白崎伸隆, 丸林拓也, 村井一真, 松下拓, 松井佳彦
  • Organizer: 第51回環境工学研究フォーラム
  • Place of Presentation: 山梨大学, 山梨県甲府市
  • Year and Date: 2014-12-21
• [Presentation] ウイルスによる水系感染症の制御に向けた浄水処理技術の高度・高効率化 (2014)
  • Author(s): 白崎伸隆, 松下拓, 松井佳彦
  • Organizer: 第22回衛生工学シンポジウム
  • Place of Presentation: 北海道大学, 北海道札幌市
  • Year and Date: 2014-11-21
• [Presentation] 腸管アデノウイルスの凝集処理性 (2014)
  • Author(s): 村井一真, 白崎伸隆, 松井佳彦, 松下拓
  • Organizer: 平成26年度全国会議 (水道研究発表会)
  • Place of Presentation: ポートメッセなごや, 愛知県名古屋市
  • Year and Date: 2014-10-31
• [Presentation] Development of novel high-basicity polyaluminum chloride for effective virus removal (2014)
  • Author(s): Marubayashi, T., Shirasaki, N., Matsushita, T., Matsui, Y. and Oshiba, A.
  • Organizer: IWA World Water Congress and Exhibition
  • Place of Presentation: Lisbon Congress Centre, Lisbon, Portugal
  • Year and Date: 2014-09-23
• [Presentation] Estimating norovirus removal performance in an in-line coagulation-ceramic microfiltration process by using recombinant norovirus VLPs and immuno-PCR method
  • Author(s): Shirasaki, N., Matsushita, T., Tatsuki, Y. and Matsui, Y.
  • Organizer: 7th IWA Specialised Membrane Technology Conference and Exhibition for Water and Wastewater Treatment and Reuse
  • Place of Presentation: Westin Harbour Castle, Toronto, Canada
• [Presentation] 全国の水道原水を用いた水系感染症ウイルスの凝集処理性評価及びウイルス処理性指標の模索
  • Author(s): 丸林拓也, 白崎伸隆, 松下拓, 松井佳彦
  • Organizer: 第48回日本水環境学会年会
  • Place of Presentation: 東北大学, 宮城県仙台市
• [Presentation] ウイルス様粒子とImmuno-PCR法を併用したノロウイルスの膜ろ過処理性評価及び処理メカニズムの解明
  • Author(s): 白崎伸隆, 田附雄一, 松下拓, 松井佳彦
  • Organizer: 第50回環境工学研究フォーラム
  • Place of Presentation: 北海道大学, 北海道札幌市
• [Presentation] インライン凝集-セラミック膜ろ過処理によるノロウイルス粒子の効果的除去
  • Author(s): 白崎伸隆, 田附雄一, 松下拓, 松井佳彦
  • Organizer: 土木学会平成25年度全国大会, 第68回年次学術講演会
  • Place of Presentation: 日本大学, 千葉県習志野市