| Budget Amount *help |
¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
Photoautotrophic fatty acid production of a highly CO2-tolerant green alga Chlorococcum littorale in the presence of inorganic carbon at 295 K and light intensity of 170 mmolphoton m-2 s-1 was investigated. CO2 concentration in the bubbling gas was adjusted by mixingpure gas components of CO2 and N2 to avoid photorespiration and b-oxidation of fatty acids under O2 surrounding conditions. Maximum content of total fatty acid showed pHdependence
after nitrate depletion of the culture media and increased with the corresponding inorganic carbon ratio. Namely, [HCO32]/([CO2]1n[CO223]) ratio in the culture media was found to be a controlling factor for photoautotrophic fatty acid production after the nitrate limitation. At a CO2 concentration of 5% (vol/vol) and a pH of 6.7, the fatty acid content was 47.8 wt % (dry basis) at its maximum that is comparable with land plant seed oils. On the other hand, simple extraction methods were also developped in this work.
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