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Proteomic analysis of citrullinated targets regulated by the p53-PADI4 pathway

Research Project

Project/Area Number 25710009
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Tumor biology
Research InstitutionThe University of Tokyo

Principal Investigator

Tanikawa Chizu  東京大学, 医科学研究所, 助教 (30422421)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2014: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2013: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywordsp53 / シトルリン化 / PADI4
Outline of Final Research Achievements

Although recent proteome analyses have provided a comprehensive overview of various posttranscriptional modifications, the functions associated with protein citrullination remain unclear. Thus, we performed a proteomic screen and identified more than 100 PADI4 substrates associated with RNA processing and translation. Citrullination of RBMX inhibited the interaction with other hnRNP family members. RNA sequence analyses revealed that the splicing patterns of more than 300 genes were disrupted in bone marrow derived from Padi4-/- mice. Citrullination at the RGG motifs was elevated in a number of cancer tissues, and an antibody against citrullinated RGG motifs was observed in 17.5% of serum samples from patients with HCV-induced HCC. Our findings revealed that PADI4-mediated citrullination is associated with RNA processing and tumor immunogenicity and suggest that PADI4 activation has potential therapeutic implications for neurodegenerative disease treatment and cancer immunotherapy.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Cystatin C as a p53‐inducible apoptotic mediator which regulates Cathepsin L activity2016

    • Author(s)
      Jinichi Mori, Chizu Tanikawa, Yuki Funauchi, Paulisally Hau Yi Lo, Yusuke Nakamura, Koichi Matsuda
    • Journal Title

      Cancer science

      Volume: 107 Issue: 3 Pages: 298-306

    • DOI

      10.1111/cas.12881

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Regulation of myo-inositol biosynthesis by p53-ISYNA1 pathway2016

    • Author(s)
      Tomoyuki Koguchi, Chizu Tanikawa, Jinichi Mori, Yoshiyuki Kojima, Koichi Matsuda
    • Journal Title

      International Journal of Oncology

      Volume: 印刷中 Issue: 6 Pages: 2415-24

    • DOI

      10.3892/ijo.2016.3456

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation of iron homeostasis by the p53-ISCU pathway2015

    • Author(s)
      Yuki Funauchi, Chizu Tanikawa, Paulisally Hau Yi Lo, Jinichi Mori, Yataro Daigo, Atsushi Takano, Yohei Miyagi, Atsushi Okawa, Yusuke Nakamura, Koichi Matsuda
    • Journal Title

      Scientific reports

      Volume: 5 Issue: 1 Pages: 16497-16497

    • DOI

      10.1038/srep16497

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Genome-wide association study identified SNP on 15q24 associated with bladder cancer risk in Japanese population.2015

    • Author(s)
      Matsuda K, Takahashi A, Middlebrooks CD, Obara W, Nasu Y, Inoue K, Tamura K, Yamasaki I, Naya Y, Tanikawa C, Cui R, Figueroa JD, Silverman DT, Rothman N, Namiki M, Tomita Y, Nishiyama H, Kohri K, Deguchi T, Nakagawa M, Yokoyama M, Miki T, Kumon H, Fujioka T, Prokunina-Olsson L, Kubo M, Nakamura Y, Shuin T.
    • Journal Title

      Hum Mol Genet.

      Volume: 24 Issue: 4 Pages: 1177-1184

    • DOI

      10.1093/hmg/ddu512

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel epigenetically inactivated gene PAMR1 in breast carcinoma.2015

    • Author(s)
      Lo PH, Tanikawa C, Katagiri T, Nakamura Y, Matsuda K.
    • Journal Title

      Oncol Rep

      Volume: 33 Issue: 1 Pages: 267-273

    • DOI

      10.3892/or.2014.3581

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Late Cornified Envelope Group I, a novel target of p53, regulates PRMT5 activity.2014

    • Author(s)
      Deng Z, Matsuda K, Tanikawa C, Lin J, Furukawa Y, Hamamoto R, Nakamura Y.
    • Journal Title

      Neoplasia

      Volume: 16 Issue: 8 Pages: 656-664

    • DOI

      10.1016/j.neo.2014.07.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of the tumor suppressor HSPB7, involved in the p53 pathway, in renal cell carcinoma by hypermethylation.2014

    • Author(s)
      Lin J, Deng Z, Tanikawa C, Shuin T, Miki T, Matsuda K, Nakamura Y.
    • Journal Title

      Int J Oncol

      Volume: 44 Issue: 5 Pages: 1490-1498

    • DOI

      10.3892/ijo.2014.2314

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of PSCA variation on gastric ulcer susceptibility.2013

    • Author(s)
      C. Tanikawa, K. Matsuo, M. Kubo, A. Takahashi, H. Ito, H. Tanaka, Y. Yatabe, K. Yamao, N. Kamatani, K. Tajima, Y. Nakamura, K. Matsuda
    • Journal Title

      Plos one

      Volume: 8 Issue: 5 Pages: e63698-e63698

    • DOI

      10.1371/journal.pone.0063698

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome wide association study of age at menarche in the Japanese population2013

    • Author(s)
      Tanikawa C, Okada Y, Takahashi A, Oda K, et al
    • Journal Title

      PLoS One

      Volume: 8 Issue: 5 Pages: e63821-e63821

    • DOI

      10.1371/journal.pone.0063821

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a Functional Variant in the MICA Promoter Which Regulates MICA Expression and Increases HCV-Related Hepatocellular Carcinoma Risk.2013

    • Author(s)
      Lo PH, Urabe Y, Kumar V, Tanikawa C, Koike K, Kato N, Miki D, Chayama K, Kubo M, Nakamura Y, Matsuda K.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 4 Pages: e61279-e61279

    • DOI

      10.1371/journal.pone.0061279

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] p53による転写制御の組織網羅的解析2015

    • Author(s)
      谷川千津、張、井元、山口、宮野、中川英刀、中村祐輔、松田浩一
    • Organizer
      日本癌学会学術集会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Regulation of RNA processing by PADI4 pathway2015

    • Author(s)
      Chizu Tanikawa, Koji Ueda, Yusuke Nakamura, Koichi Matsuda.
    • Organizer
      AACR Annual Meeting
    • Place of Presentation
      Philadelphia, Pennsylvania, USA
    • Year and Date
      2015-04-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of RNA processing by p53-PADI4 pathway2014

    • Author(s)
      谷川千津、植田幸嗣、中川英刀、中村祐輔、松田浩一
    • Organizer
      日本癌学会学術集会
    • Place of Presentation
      パシフィコ横浜、神奈川県横浜市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Proteomic analysis of citrullinated targets regulated by the p53-PADI4 pathway2014

    • Author(s)
      Chizu Tanikawa, Koji Ueda, Hidewaki Nakagawa, Yusuke Nakamura, Koichi Matsuda.
    • Organizer
      American Association for Cancer Research
    • Place of Presentation
      San Diego, California, USA
    • Year and Date
      2014-04-05 – 2014-04-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] Proteomic analysis of citrullinated targets regulated by the p53-PADI4 pathway2013

    • Author(s)
      谷川千津、植田幸嗣、中川英刀、中村祐輔、松田浩一
    • Organizer
      日本癌学会学術集会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] A genome-wide association study identifies two susceptibility loci for duodenal ulcer in the Japanese population2013

    • Author(s)
      Chizu Tanikawa, Koichi Matsuda, Yusuke Nakamura
    • Organizer
      American Association for Cancer Research
    • Place of Presentation
      Washington, DC, USA
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-21   Modified: 2019-07-29  

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