Proteomics analysis of cancer stem cell and its microenvironment using FFPE tissue and cancer cell line

• Project/Area Number: 25710013
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Shizuoka Cancer Center Research Institute
• Principal Investigator: Hatakeyama Keiichi 静岡県立静岡がんセンター(研究所), その他部局等, その他 (20564157)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000); Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: プロテオミクス解析 / 単一細胞解析 / がん / プロテオミクス / 微小環境 / がん幹細胞 / FFPE組織

Outline of Final Research Achievements

This research aim is discovery of molecules for prognostic prediction using proteome analysis of cancer stem cell and its microenvironment. This approach focuses on analysis of formalin-fixed paraffin-embedded (FFPE) tissue and cancer cell line.
We identified proteins secreted from unique cell population in colorectal cancer. Furthermore, this achievement progressed a new research to analyze single cancer cell, resulting wining next grant-in-aid.

Research Products

• [Journal Article] A novel splice variant of XIAP-associated factor 1 (XAF1) is expressed in peripheral blood containing gastric cancer-derived circulating tumor cells (2015)
  • Author(s): Hatakeyama, K., Yamakawa, Y., Fukuda, Y., Ohshima, K., Wakabayashi-Nakao, K., Sakura, N., Tanizawa, Y., Kinugasa, Y., Yamaguchi, K., Terashima, M., Mochizuki, T
  • Journal Title: Gastric Cancer Volume: 18 Issue: 4 Pages: 751-761
  • DOI: 10.1007/s10120-014-0426-3
  • Peer Reviewed
• [Presentation] Gene expression profiling of Japanese gastric cancer using Asian Cancer Research Group classification (2016)
  • Author(s): Masanori Terashima, Rie Makuuchi, Masanori Tokunaga, Yutaka Tanizawa, Etsuro Bando, Taiichi Kawamura, Makoto Hikage, Keiichi Hatakeyama, Keiichi Ohshima, Shumpei Ohnami, Kennich Urakami, Masatoshi Kusuhara, Ken Yamaguchi
  • Organizer: 2016 Gastrointestinal Cancers Symposium
  • Place of Presentation: Moscone West Building, Sun Francisco, CA
  • Year and Date: 2016-01-21
  • Int'l Joint Research
• [Presentation] Whole exome sequencing and gene expression profiling of neuroendocrine carcinoma in the stomach (2016)
  • Author(s): Rie Makuuchi, Masanori Terashima, Keiichi Hatakeyama, Masanori Tokunaga, Yutaka Tanizawa, Etsuro Bnado, Taiichi Kawamura, Keiichi Ohshima, Kenichi Urakami, Masatoshi Kusuhara, Ken Yamaguchi
  • Organizer: 2016 Gastrointestinal Cancers Symposium
  • Place of Presentation: Moscone West Building, Sun Francisco, CA
  • Year and Date: 2016-01-21
  • Int'l Joint Research
• [Presentation] Genomic profiling of gastric cancer using High-tech Omics-based Patient Evaluation (HOPE) (2015)
  • Author(s): Rie Makuuchi, Masanori Terashima, Shinsaku Honda, Taichi Tatsubayashi, Makoto Hikage, Masanori Tokunaga, Yutaka Tanizawa, Etsuro Bando, Taiichi Kawamura, Keiichi Hatakeyama, Keiichi Oshima, Kennich Urakami, Masatoshi Kusuhara, Ken Yamaguchi
  • Organizer: 第53回癌治療学会学術集会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都
  • Year and Date: 2015-10-29
• [Presentation] CEA family profiling in 1,000 cancer patients based on somatic mutations associated with gene expression alteration (2015)
  • Author(s): 畠山慶一、大島啓一、長嶋剛史、大浪澄子、大浪俊平、丸山宏二、秋山靖人、浦上研一、楠原正俊、山口建、望月徹
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-10-08
• [Presentation] Gene expression profiling: a single institution study of 1,000 cancer patients (2015)
  • Author(s): 大島啓一、長嶋剛史、畠山慶一、下田勇治、田邉智絵、大浪澄子、大浪俊平、芹澤昌邦、浦上研一、秋山靖人、楠原正俊、望月徹、山口建
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-10-08
• [Presentation] Prognostic significance of survivin expression in patients with gastric cancer (2014)
  • Author(s): M.Terashima, K. Hatakeyama, Y. Yamakawa, Y. Miki, R. Makuuchi, S. Honda, T. Tatsubayashi, M. Tokunaga, Y. Tanizawa, E. Bando, T. Kawamura, K. Oshima, T. Mochizuki
  • Organizer: European Society for Medical Oncology
  • Place of Presentation: Madrid, Spain
  • Year and Date: 2014-09-26 – 2014-09-30
• [Presentation] Novel splice variant of XIAP-associated factor 1 (XAF1) is expressed in peripheral blood of patient with gastric cancer (2014)
  • Author(s): Keiichi Hatakeyama, Yushi Yamakawa, Keiichi Ohshima, Kanako Nakao, Yutaka Tanizawa, Yusuke Kinugasa, Ken Yamaguchi, Masanori Terashima, Tohru Mochizuki
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 神奈川県, 横浜市
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Survivin expression in peripheral blood is a significant prognostic marker in patients with gastric cancer (2014)
  • Author(s): M.Terashima, K. Hatakeyama, Y. Yamakawa, N. Sugisawa, Y. Miki, R. Makuuchi, H. Goto, M. Tokunaga, Y. Tanizawa, E. Bando, T. Kawamura, K. Oshima, T. Mochizuki
  • Organizer: American Society of Clinical Oncology
  • Place of Presentation: Chicago, IL, USA
  • Year and Date: 2014-05-30 – 2014-06-03
• [Presentation] Survivin expression in circulating tumor cells of patients with gastric cancer (2014)
  • Author(s): Masanori Terashima, Keiichi Hatakeyama, Yushi Yamakawa, Norihiko Sugisawa, Yuichiro Miki, Rie Makuuchi, Hironobu Goto, Masanori Tokunaga, Yutaka Tanizawa, Etsuro Bando, Taiichi Kawamura, Keiichi Oshima, Toru Mochizuki
  • Organizer: 2014 ASCO Gastrointestinal Cancers Symposium
  • Place of Presentation: San Francisco, California, USA
• [Presentation] Identification of novel protein isoforms in secretome using combined analysis of microarray and mass spectrometry (2014)
  • Author(s): Keiichi Hatakeyama, Keiichi Ohshima, Kanako Wakabayashi-Nakao, Yutaka Tanizawa, Masanori Terashima, Ken Yamaguchi and Tohru Mochizuki
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 神奈川県, パシフィコ横浜
• [Patent(Industrial Property Rights)] 甲状腺髄様がん判定方法 (2013)
  • Inventor(s): 中尾香菜子, 望月徹, 畠山慶一
  • Industrial Property Rights Holder: 中尾香菜子, 望月徹, 畠山慶一
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-04-25
  • Acquisition Date: 2013-12-16