Study on the splicing checkpoint mechanism to prevent pre-mRNA accumulation caused by splicing abnormality

• Project/Area Number: 25711001
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Molecular biology
• Research Institution: University of Toyama
• Principal Investigator: Kaida Daisuke 富山大学, 大学院医学薬学研究部(医学), 准教授 (60415122)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000); Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: スプライシング / 転写 / RNAポリメラーゼII

Outline of Final Research Achievements

mRNA splicing is a fundamental mechanism for the gene expression in eukaryotes. Splicing abnormality might cause pre-mRNA accumulation and diseases. Therefore, I assumed that cells have a checkpoint mechanism that detects splicing abnormality and suppresses transcriptional elongation to prevent pre-mRNA accumulation. I revealed that such a splicing checkpoint mechanism actually exists in this study.

Research Products

• [Journal Article] Upregulation of p27 cyclin-dependent kinase inhibitor and a C-terminus truncated form of p27 contributes to G1 phase arrest (2016)
  • Author(s): Takayuki Satoh, Daisuke Kaida
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 27829-27829
  • DOI: 10.1038/srep27829
  • Peer Reviewed / Open Access
• [Journal Article] The reciprocal regulation between splicing and 3′‐end processing (2016)
  • Author(s): Daisuke Kaida
  • Journal Title: Wiley Interdisciplinary Reviews: RNA Volume: 7 Issue: 4 Pages: 499-511
  • DOI: 10.1002/wrna.1348
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Splicing inhibition decreases phosphorylation level of Ser2 in Pol II CTD (2015)
  • Author(s): Mitsunori Koga, Megumi Hayashi and Daisuke Kaida
  • Journal Title: Nucleic Acids Research Volume: 43 Issue: 17 Pages: 8258-8267
  • DOI: 10.1093/nar/gkv740
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] U2 snRNP is required for expression of the 3' end of genes (2014)
  • Author(s): Koga, M., Satoh, T., Takasaki, I., Kawamura, Y., Yoshida, M., and Kaida, D
  • Journal Title: PloS One Volume: 9 Issue: 5 Pages: e98015-e98015
  • DOI: 10.1371/journal.pone.0098015
  • Peer Reviewed
• [Presentation] 細胞の正常な機能を維持するmRNAスプライシングチェックポイント機構 (2017)
  • Author(s): 甲斐田大輔
  • Organizer: 富山RNAワークショップ
  • Place of Presentation: 富山大学
  • Year and Date: 2017-03-13
  • Invited
• [Presentation] 細胞の正常な機能を維持するmRNAスプライシングチェックポイント機構 (2017)
  • Author(s): 甲斐田大輔
  • Organizer: 第３回バイオシグナル研究会
  • Place of Presentation: 神戸大学
  • Year and Date: 2017-03-08
  • Invited
• [Presentation] スプライシング阻害剤スプライソスタチンAは転写活性と細胞周期進行を制御する (2015)
  • Author(s): 甲斐田大輔
  • Organizer: 第38回 日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] mRNAスプライシングの異常はPol II Ser2の脱リン酸化を引き起こす (2015)
  • Author(s): 古賀光徳、甲斐田大輔
  • Organizer: 第２回北陸エピジェネティクス研究会
  • Place of Presentation: 富山
  • Year and Date: 2015-11-11
• [Presentation] Splicing inhibition decreases phosphorylation level of Pol II CTD Ser2 (2015)
  • Author(s): Daisuke Kaida and Mitsunori Koga
  • Organizer: RNA 2015 The 20th Annual Meeting of the RNA Society
  • Place of Presentation: Madison, WI, USA
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Presentation] スプライシング活性はPol II Ser2のリン酸化と転写伸長に必要である (2014)
  • Author(s): 古賀光徳, 佐藤崇之, 甲斐田大輔
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] mRNA splicing activity is required for efficient transcription elongation (2014)
  • Author(s): 甲斐田大輔
  • Organizer: RIKEN Symposium/15th Tokyo RNA Club “Noncoding RNA regulation“
  • Place of Presentation: 埼玉
  • Year and Date: 2014-10-01
  • Invited
• [Presentation] スプライシング活性はPol II Ser2のリン酸化と転写伸長に必要である (2014)
  • Author(s): 古賀光徳, 佐藤崇之, 甲斐田大輔
  • Organizer: 第１６回日本RNA学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2014-07-23 – 2014-07-25
• [Presentation] U2 snRNP is required for transcription elongation in a gene specific manner. (2014)
  • Author(s): 古賀光徳, 佐藤崇之, 甲斐田大輔
  • Organizer: RNA 2014 The 19th Annual Meeting of the RNA Society
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2014-06-03 – 2014-06-08
• [Presentation] U2 snRNP is required for the phosphorylation of Pol II CTD and efficient transcription elongation. (2014)
  • Author(s): 古賀光徳, 佐藤崇之, 甲斐田大輔
  • Organizer: 2014 American Society for Biochemistry and Molecular Biology Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2014-04-26 – 2014-04-30
• [Presentation] スプライシング異常はP-TEFbを阻害することで転写伸長抑制を引き起こす (2013)
  • Author(s): 古賀光徳、甲斐田大輔
  • Organizer: 日本RNA学会年会
  • Place of Presentation: 愛媛県県民文化会館
• [Presentation] U2 snRNP is required for the phosphorylation of CTD Ser2 and efficient transcription elongation (2013)
  • Author(s): Daisuke Kaida, Mitsunori Koga
  • Organizer: Cold Spring Harbor Laboratory meeting “Eukaryotic mRNA processing”
  • Place of Presentation: Cold Spring Harbor Laboratory
• [Book] ノンコーディングRNA (2016)
  • Author(s): 甲斐田大輔
  • Total Pages: 355
  • Publisher: 化学同人