| Budget Amount *help |
¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
Cohesin plays a crucial role in sister chromatid cohesion and gene expression. The dynamic association of cohesin with chromatin is essential for these functions. However, the exact nature of cohesin dynamics remains unclear. In this study I evaluated the dynamics of individual cohesin molecules on DNA and found that the cohesin possesses an intrinsic ability to traverse DNA in an ATPase-dependent manner. Translocation ability is suppressed in the presence of Wapl-Pds5 and Sororin; this suppression is alleviated by the acetylation of cohesin and the action of mitotic kinases. In Xenopus egg extracts, cohesin is translocated on DNA in an ATPase- and Smc3 acetylation-dependent manner. Cohesin movement changes to unidirectional when cohesin faces DNA replication; otherwise, it is incorporated into replicating DNA. This study provides insight into the nature of cell cycle-dependent changes of cohesin dynamics, and also evolutionally conserved mechanisms to regulate cohesin dynamics.
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