| Budget Amount *help |
¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Outline of Final Research Achievements |
Separase autocleavage was required for its sharp activation during mitosis. We found that instead of promoting separase activation, autocleavage maintained separase in inactive state until the onset of anaphase, by regulating binding of its inhibitor cyclin B1. This tight regulation of separase was widely abrogated in many cancer cell lines, which was highly correlated with longer mitosis. Treating RPE1-a widely used normal diploid cell line, with a low dose of nocodazole, prolonged mitosis and resulted in perturbed tight regulation of separase and frequent chromosome missegregation. Longer mitosis and subsequent deregulation of separase could provide explanation for the frequent chromosome missegregation in cancer cells.
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