Understanding chromosome segregation using Separase biosensor
| Project/Area Number |
25711003
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Japanese Foundation for Cancer Research |
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Principal Investigator |
Shindo Norihisa 公益財団法人がん研究会, がん研究所 実験病理部, 研究員 (00512253)
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| Project Period (FY) |
2013-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥26,390,000 (Direct Cost: ¥20,300,000、Indirect Cost: ¥6,090,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
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| Keywords | 染色体分離 / 細胞分裂 / セパレース / がん細胞株 / 染色体 / バイオセンサー / 数理的モデリング / 染色体分配 / 活性制御 |
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| Outline of Final Research Achievements |
Separase autocleavage was required for its sharp activation during mitosis. We found that instead of promoting separase activation, autocleavage maintained separase in inactive state until the onset of anaphase, by regulating binding of its inhibitor cyclin B1. This tight regulation of separase was widely abrogated in many cancer cell lines, which was highly correlated with longer mitosis. Treating RPE1-a widely used normal diploid cell line, with a low dose of nocodazole, prolonged mitosis and resulted in perturbed tight regulation of separase and frequent chromosome missegregation. Longer mitosis and subsequent deregulation of separase could provide explanation for the frequent chromosome missegregation in cancer cells.
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Report
(6 results)
Research Products
(21 results)
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[Presentation] 進藤 軌久、 広田 亨2016
Author(s)
がん細胞における染色体分離制御の時空間特性
Organizer
第75回 日本癌学会学術総会
Place of Presentation
神奈川県、パシフィコ横浜
Year and Date
2016-10-06
Related Report
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