| Budget Amount *help |
¥26,650,000 (Direct Cost: ¥20,500,000、Indirect Cost: ¥6,150,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
In this research project, we aimed to unravel the phenomenological laws that impose the constraints on growth and death of individual cells under constant culture conditions, using E. coli, S. pombe, and L1210 lymphocytic leukemia cells as model organisms. The study revealed the linear relation between the means and the variances of E. coli generation time, which might determine the maximum bound of growth rate of this organism in any constant environments. Long-term single-cell measurement of S. pombe revealed the linear relation between division rate and death rate in constant environments, which demonstrates the trade-off that faster growth is linked with even higher death rates at the single-cell level. We also developed a new microfluidic device for long-term single-cell measurements of L1210 cells.
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