Analysis of the mechanism that creates the polarity and separates germ layer fates.
Project/Area Number |
25711016
|
Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Partial Multi-year Fund |
Research Field |
Developmental biology
|
Research Institution | Tokyo Metropolitan University |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
|
Keywords | 細胞極性 / 胚葉運命 / 核移動 / PI3K / 胚葉運命分離 / 中胚葉 / 内胚葉 / mRNA局在 / 発生生物学 / 核 |
Outline of Final Research Achievements |
I analyzed the mechanism responsible for creation of diverse types of cells that constitute the animal body from a single cell, the fertilized egg. The initial stages of this process is the formation of the germ layer cells. In previous studies, I found that mesoderm and endoderm fates are separated by nuclear migration-dependent localization of a mRNA encoding Not within the mesendoderm cell and its partitioning to the mesoderm daughter cell. In this study, I analyzed the mechanism that determines the direction of the nuclear migration, as it is central to fate separation. I found that localization of PI3K, a membrane protein, to the future mesoderm side of the mesendoderm cell determines the direction of nuclear migration. Localization of PI3K was dependent on actin dependent re-localization of egg cytoplasm immediately after fertilization. The localization was maintained by activation of PI3K in the future mesoderm region at the 4-cell stage.
|
Report
(4 results)
Research Products
(14 results)
-
-
[Journal Article] ANISEED 2015: a digital framework for the comparative developmental biology of ascidians.2015
Author(s)
Brozovic, Martin, Dantec, Dauga, Mendez, Simion, Percher, Laporte, Scornavacca, Di Gregorio, Fujiwara, et al.
-
Journal Title
Nucleic Acids Research
Volume: 44
Issue: D1
Pages: 808-818
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-