| Budget Amount *help |
¥26,780,000 (Direct Cost: ¥20,600,000、Indirect Cost: ¥6,180,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
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| Outline of Final Research Achievements |
I analyzed the mechanism responsible for creation of diverse types of cells that constitute the animal body from a single cell, the fertilized egg. The initial stages of this process is the formation of the germ layer cells. In previous studies, I found that mesoderm and endoderm fates are separated by nuclear migration-dependent localization of a mRNA encoding Not within the mesendoderm cell and its partitioning to the mesoderm daughter cell. In this study, I analyzed the mechanism that determines the direction of the nuclear migration, as it is central to fate separation. I found that localization of PI3K, a membrane protein, to the future mesoderm side of the mesendoderm cell determines the direction of nuclear migration. Localization of PI3K was dependent on actin dependent re-localization of egg cytoplasm immediately after fertilization. The localization was maintained by activation of PI3K in the future mesoderm region at the 4-cell stage.
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