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Plant stomata developmental regulation in response to elevated CO2

Research Project

Project/Area Number 25711017
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionThe University of Tokyo

Principal Investigator

Higaki Takumi  東京大学, 新領域創成科学研究科, 特任准教授 (90578486)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords気孔 / 二酸化炭素 / 葉表皮細胞 / バイオイメージング / シロイヌナズナ / 形態計測 / 画像クラスタリング / 数理モデル
Outline of Final Research Achievements

Plants regulate guard cell differentiation in response to environmental cues including CO2 levels. Some reports have shown that elevated CO2 concentrations suppress stomatal development, but other studies could not reproduce the effect. To clarify this issue, quantitative imaging analysis of stomatal pattern formation was conducted with Arabidopsis cotyledons grown under ambient and elevated CO2 conditions. Our microscopic image analysis revealed that elevated CO2 did not affect stomatal density/index but perturbed the uniform distribution of stomata, with excess satellite stomata and meristemoids. We then used an overexpression line of a gene for a DNA replication licensing factor, CDC6, which was reported to positively regulate satellite meristemoid production. The overexpressor showed hypersensitivity to elevated CO2-induced stomatal distribution changes, suggesting that elevated CO2 positively regulates CDC6-mediated satellite stomata production, at least in Arabidopsis cotyledons.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (46 results)

All 2017 2016 2015 2014 2013 Other

All Journal Article (19 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 19 results,  Open Access: 13 results,  Acknowledgement Compliant: 8 results) Presentation (20 results) (of which Invited: 4 results) Remarks (7 results)

  • [Journal Article] Exogenous cellulase switches cell interdigitation to cell elongation in a RIC1-dependent manner in Arabidopsis thaliana cotyledon pavement cells.2017

    • Author(s)
      Higaki T, Takigawa-Imamura H, Akita K, Kutsuna N, Kobayashi R, Hasezawa S, Miura T
    • Journal Title

      Plant Cell Physiol

      Volume: 58 Pages: 106-119

    • DOI

      10.1093/pcp/pcw183

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] SMALL ACIDIC PROTEIN 1 (SMAP1) and SCFTIR1 ubiquitin proteasome pathway act in concert to induce 2,4-dichlorophenoxyacetic acid-mediated alteration of actin in Arabidopsis roots.2017

    • Author(s)
      Takahashi M, Umetsu K, Oono Y, Higaki T, Blancaflor E, Rahman A
    • Journal Title

      Plant J

      Volume: 89 Issue: 5 Pages: 940-956

    • DOI

      10.1111/tpj.13433

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Theoretical Model of Jigsaw-Puzzle Pattern Formation by Plant Leaf Epidermal Cells2016

    • Author(s)
      Takumi Higaki, Natsumaro Kutsuna, Kae Akita, Hisako Takigawa-Imamura, Kenji Yoshimura, Takashi Miura
    • Journal Title

      PLOS Computational Biology

      Volume: 12 Issue: 4 Pages: e1004833-e1004833

    • DOI

      10.1371/journal.pcbi.1004833

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantitative evaluation of stomatal cytoskeletal patterns during the activation of immune signaling in Arabidopsis thaliana.2016

    • Author(s)
      M. Shimono, T. Higaki, H. Kaku, N. Shibuya, S. Hasezawa, B. Day
    • Journal Title

      PLoS ONE

      Volume: 11 Issue: 7 Pages: e0159291-e0159291

    • DOI

      10.1371/journal.pone.0159291

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote.2016

    • Author(s)
      Kimata, Y., Higaki, T., Kawashima, T., Kurihara, D., Sato, Y., Yamada, T., Hasezawa, S., Berger, F., Higashiyama, T., and Ueda, M.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 113 Issue: 49 Pages: 14157-14162

    • DOI

      10.1073/pnas.1613979113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Dominant and recessive mutations in the Raf-like kinase HT1 gene completely disrupt stomatal responses to CO2.2016

    • Author(s)
      Hashimoto-Sugimoto, M., Negi, J., Monda, K., Higaki, T., Isogai, Y., Nakano, T., Hasezawa, S. and Iba, K.
    • Journal Title

      J.Exp. Bot.

      Volume: 未定 Issue: 11 Pages: 3251-3261

    • DOI

      10.1093/jxb/erw134

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Nuclear function of subclass I actin depolymerizing factor contributes to susceptibility in Arabidopsis to an adapted powdery mildew fungus.2016

    • Author(s)
      Inada N, Higaki T, Hasezawa S
    • Journal Title

      Plant Physiol

      Volume: 170 Issue: 3 Pages: 1420-1434

    • DOI

      10.1104/pp.15.01265

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Quantitative analyses on dynamic changes in the organization of host Arabidopsis thaliana actin microfilaments surrounding the infection organ of the powdery mildew fungus Golovinomyces orontii.2016

    • Author(s)
      Inada N, Higaki T, Hasezawa S
    • Journal Title

      J Plant Res

      Volume: 129 Issue: 1 Pages: 103-110

    • DOI

      10.1007/s10265-015-0769-9

    • NAID

      40020697794

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Semi-automatic organelle detection on transmission electron microscopic images2015

    • Author(s)
      T. Higaki, N. Kutsuna, K. Akita, M. Sato, F. Sawaki, M. Kobayashi, N. Nagata, K. Toyooka, S. Hasezawa
    • Journal Title

      SCIENTIFIC REPORTS

      Volume: 5 Issue: 1 Pages: 7794-7794

    • DOI

      10.1038/srep07794

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora.2015

    • Author(s)
      Hirakawa Y, Nomura T, Hasezawa S, Higaki T
    • Journal Title

      Journal of Integrated Plant Biology

      Volume: 57 Issue: 1 Pages: 127-135

    • DOI

      10.1111/jipb.12304

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Radiocesium distribution in bamboo shoots after the Fukushima nuclear accident.2014

    • Author(s)
      Higaki T, Higaki S, Hirota M, Hasezawa S
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 5 Pages: e97659-e97659

    • DOI

      10.1371/journal.pone.0097659

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log to stationary transition of growth phase in tobacco BY-2 cells.2014

    • Author(s)
      Toyooka K, Sato M, Kutsuna N, Higaki T, Sawaki F, Wakazaki M, Goto Y, Hasezawa S, Nagata N, Matsuoka K
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 9 Pages: 1544-1555

    • DOI

      10.1093/pcp/pcu084

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increase in Invaginated Vacuolar Membrane Structure Caused by Plant Cell Expansion by Genotoxic Stress Induced by DNA Double-Strand Breaks2014

    • Author(s)
      Hasegawa J, Higaki T, Hamamura Y, Kurihara D, Kutsuna N, Higashiyama T, Hasezawa S, Matsunaga S
    • Journal Title

      CYTOLOGIA

      Volume: 79 Issue: 4 Pages: 467-474

    • DOI

      10.1508/cytologia.79.467

    • NAID

      130004801651

    • ISSN
      0011-4545, 1348-7019
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamics and environmental responses of PATROL1 in Arabidopsis subsidiary cells.2014

    • Author(s)
      Higaki, T., Hashimoto-Sugimoto, M., Akita, K., Iba, K., and Hasezawa, S.
    • Journal Title

      Plant Cell Physiol.

      Volume: 55 Issue: 4 Pages: 773-780

    • DOI

      10.1093/pcp/pct151

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Investigation of contamination in spinach collected immediately following the Fukushima Daiichi nuclear disaster2013

    • Author(s)
      Hirota M, Higaki S, Higaki T, Hasezawa S
    • Journal Title

      Radiation Safety Management

      Volume: 12 Issue: 2 Pages: 43-47

    • DOI

      10.12950/rsm.12.43

    • NAID

      130003388422

    • ISSN
      1347-1511, 1884-9520
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Breaking of the plant stomatal one-cell-spacing rule by sugar solution immersion.2013

    • Author(s)
      Akita K, Hasezawa S, Higaki T
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 9 Pages: e72456-e72456

    • DOI

      10.1371/journal.pone.0072456

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles of cortical actin microfilament patterning in division plane orientation in plants.2013

    • Author(s)
      Kojo KH, Higaki T, Kutsuna N, Yoshida Y, Yasuhara H, Hasezawa S
    • Journal Title

      Plant and Cell Physiology

      Volume: 54 Issue: 9 Pages: 1491-1503

    • DOI

      10.1093/pcp/pct093

    • NAID

      10031202071

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses2013

    • Author(s)
      Hashimoto-Sugimoto, M., Higaki, T., Yaeno, T., Nagami, A., Irie, M., Fujimi M., Miyamoto, M., Akita, K., Negi, J., Shirasu, K., Hasezawa, S. and Iba, K.
    • Journal Title

      Nature Commun.

      Volume: 4 Issue: 1 Pages: 2215-2215

    • DOI

      10.1038/ncomms3215

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] LIPS database with LIPService: a microscopic image database of intracellular structures in Arabidopsis guard cells.2013

    • Author(s)
      Higaki T, Kustuna N, Hasezawa S
    • Journal Title

      BMC Plant Biology

      Volume: 13 Issue: 1 Pages: 81-81

    • DOI

      10.1186/1471-2229-13-81

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] 子葉表皮細胞壁の輪局における微小管結合タンパク質RIC1の役割: 細胞形態計測と力学モデルによる解析2017

    • Author(s)
      桧垣匠、今村寿子、秋田佳恵、朽名夏麿、三浦岳、馳澤盛一郎
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞動態を理解するための画像解析とモデル解析2016

    • Author(s)
      桧垣匠
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県那覇市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] A model jigsaw puzzle pattern formation by plant leaf epidermal cell wall.2016

    • Author(s)
      Takumi Higaki, Natsumaro Kutsuna, Kae Akita, Hisako Takigawa-Imamura, Kenji Yoshimura, Seiichiro Hasezawa, Takashi Miura
    • Organizer
      The 57th Annual meeting of the Japanase Society of Plant Physiologists
    • Place of Presentation
      Iwate University(Morioka city, Iwate Prefecture)
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] シロイヌナズナ葉表皮細胞における細胞壁湾曲機構の解析2015

    • Author(s)
      桧垣 匠, 今村 寿子, 秋田 佳恵, 朽名 夏麿, 馳澤 盛一郎, 三浦 岳
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ(新潟県新潟市)
    • Year and Date
      2015-09-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物の細胞板形成を支える分子メカニズム2015

    • Author(s)
      笹部美和子,桧垣匠,大和田理恵,相田治寿,西田結花,馳澤盛一郎,町田泰則
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物プロトプラストの細胞壁再生の時間的・空間的制御機構2015

    • Author(s)
      横山隆亮,九鬼寛明,桧垣匠,竹内美和,馳澤盛一郎,西谷和彦
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 気孔開口運動における表層微小管機能に関する再検証:膜交通因子PATROL1との関係2015

    • Author(s)
      桧垣匠,橋本(杉本)美海,秋田佳恵,花俣繁,的場厚,馳澤盛一郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] タバコBY-2細胞を用いたErwinia carotovora培養濾過液誘導性細胞死における液胞単純化の観察2015

    • Author(s)
      平川由美,桧垣匠,野村俊尚,馳澤盛一郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] スクロース水浸処理によりクラスター化した孔辺細胞における細胞内構造の検討2015

    • Author(s)
      秋田佳恵,桧垣匠,馳澤盛一郎
    • Organizer
      第56回日本植物生理学会年会
    • Place of Presentation
      東京農業大学世田谷キャンパス(東京都世田谷区)
    • Year and Date
      2015-03-16 – 2015-03-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞画像認識の実際: 蛍光画像と電顕画像を例に2014

    • Author(s)
      桧垣匠
    • Organizer
      東京理科大学イメージングフロンティアシンポジウム2014
    • Place of Presentation
      東京理科大学野田キャンパス(千葉県野田市)
    • Year and Date
      2014-12-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 葉肉細胞プロトプラストの細胞壁再生系の開発とイメージング解析を利用した細胞壁構築過程の解析2014

    • Author(s)
      九鬼寛明,桧垣匠,小浜大山,横山隆亮,篠原直貴,馳澤盛一郎,西谷和彦
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 気孔複合体における膜交通制御因子PATROL1の動態解析2014

    • Author(s)
      桧垣匠,橋本(杉本)美海,秋田佳恵,射場厚,馳澤盛一郎
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA損傷ストレスにおけるオーキシンの機能解析2014

    • Author(s)
      長谷川淳子,桧垣匠,朽名夏麿,坂本卓也,綿引雅昭,馳澤盛一郎,松永幸大
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] スクロース水溶液水浸処理により形成される気孔クラスターの解析2014

    • Author(s)
      秋田佳恵,桧垣匠,馳澤盛一郎
    • Organizer
      日本植物学会第78回大会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 透過型電子顕微鏡画像に基づく葉緑体微細構造異常の自動分類2014

    • Author(s)
      桧垣匠,加藤綾,明賀史純,朽名夏麿,馳澤盛一郎,永田典子
    • Organizer
      第23回日本バイオイメージング学会学術集会
    • Place of Presentation
      大阪大学銀杏会館(大阪府吹田市)
    • Year and Date
      2014-09-04 – 2014-09-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロイヌナズナ子葉の表皮組織形成に対するセルラーゼ処理の影響2014

    • Author(s)
      桧垣匠,秋田佳恵,朽名夏麿,馳澤盛一郎
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山大学五福キャンパス(富山県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物の気孔開閉を制御する膜交通因子の動態解析2013

    • Author(s)
      桧垣匠
    • Organizer
      東京理科大学イメージングフロンティアシンポジウム2013
    • Place of Presentation
      東京理科大学野田キャンパス(千葉県)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 生細胞イメージングと画像情報処理による細胞骨格・オルガネラの動態解析2013

    • Author(s)
      桧垣匠
    • Organizer
      第22回日本バイオイメージング学会学術集会
    • Place of Presentation
      東京大学薬学部キャンパス(東京都)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 電顕画像における注目構造の自動検索法の開発2013

    • Author(s)
      桧垣匠,朽名夏麿,馳澤盛一郎
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学高等教育推進機構(北海道)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Cell Wall and Skull Bone: Understanding Pattern Formation Dynamics using Mathematical Model2013

    • Author(s)
      桧垣匠,三浦岳
    • Organizer
      第46回日本発生生物学会
    • Place of Presentation
      島根県立産業交流会館くにびきメッセ(島根県)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Remarks] 植物の細胞壁が外部刺激に応答する分子機構の一端を解明

    • URL

      http://www.k.u-tokyo.ac.jp/info/entry/7_entry46/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 植物の受精卵が偏る仕組みを発見

    • URL

      http://www.k.u-tokyo.ac.jp/info/entry/22_entry522/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 葉っぱと頭蓋骨の意外な関係? ~植物細胞の変形を説明する新理論を確立~

    • URL

      http://www.k.u-tokyo.ac.jp/info/entry/7_entry36/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 新領域創成科学研究科ニュース「省力・低コストで電子顕微鏡画像からオルガネラを検出する方法を開発」

    • URL

      http://www.k.u-tokyo.ac.jp/info/entry/7_entry24/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 新領域創成科学研究科ニュース「植物病原細菌誘導性細胞死における新たな液胞膜動態を発見」

    • URL

      http://www.k.u-tokyo.ac.jp/info/entry/7_entry23/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 植物で発見された動物の神経伝達調節因子はどのような機能をもつか

    • URL

      http://www.k.u-tokyo.ac.jp/info/entry/7_entry11/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 砂糖水で植物の気孔がくっついた: 気孔分布パターンを変化させる栽培条件の発見

    • URL

      http://www.k.u-tokyo.ac.jp/info/entry/7_entry12/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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