Molecular mechanisms of plant parasitism

• Project/Area Number: 25711019
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology (2016-2017); Institute of Physical and Chemical Research (2013-2015)
• Principal Investigator: Yoshida Satoko 奈良先端科学技術大学院大学, 研究推進機構, 特任准教授 (20450421)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000); Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2014: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2013: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 寄生植物 / 遺伝学 / 変異体 / ハマウツボ科 / ゲノム / 次世代シーケンサー / コシオガマ / 吸器形成 / 遺伝子 / 遺伝子同定 / 遺伝地図 / 原因遺伝子同定

Outline of Final Research Achievements

Parasitic plants in Orobanchaceae are noxious agricultural pests that affects millions of people's life. However the genetic programs that control plant parasitism remain unknown. In this study, we aimed to elucidate molecular mechanisms underlying plant parasitism using a model parasitic plant Phtheirospermum japonicum. We have screened P. japonicum mutants that have defects in host interaction and haustoirum formation. The genome of P. japonicum was sequenced and re-sequencing of the mutant lines successfully identified the responsible gene for the phenotype. This study provide us new tools to identify key genes in host and parasitic plant interaction.

Research Products

• [Int'l Joint Research] Pennsylvania State University/University of Virginia(米国)
• [Int'l Joint Research] Tront University(カナダ)
• [Int'l Joint Research] Penn State University/Virginia University(米国)
• [Int'l Joint Research] Seoul University(韓国)
• [Journal Article] Host lignin composition affects haustorium induction in the parasitic plants Phtheirospermum japonicum and Striga hermonthica (2018)
  • Author(s): Cui S, Wada S, Tobimatsu Y, Takeda Y, Saucet S, Takano T, Umezawa T, Shirasu K, Yoshida S
  • Journal Title: New Phytologist Volume: 218 Issue: 2 Pages: 710-723
  • DOI: 10.1111/nph.15033
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation and functional diversification of root hairs (2017)
  • Author(s): Cui, S., Suzaki, T., Tominaga-wada, R., Yoshida, S.
  • Journal Title: Sem. Cell. Dev. Biol. Volume: in press Pages: 115-122
  • DOI: 10.1016/j.semcdb.2017.10.003
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Quinone oxidoreductase 2 is involved in haustorium development of the parasitic plant Phtheirospermum japonicum (2017)
  • Author(s): Ishida JK, Yoshida S, Shirasu K.
  • Journal Title: Plant Signal Behav Volume: 12 Issue: 7 Pages: e1319029-e1319029
  • DOI: 10.1080/15592324.2017.1319029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Haustorium induction assay of the parasitic plant Phtheirospermum japonicum. (2017)
  • Author(s): Ishida, J.K., Yoshida, S., Shirasu, K.
  • Journal Title: Bio-protocol Volume: 7 Issue: 9
  • DOI: 10.21769/bioprotoc.2260
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Inter-species hormonal control of host root morphology by parasitic plants. (2017)
  • Author(s): Spallek, T., Melnyk, C.W., Wakatake, T., Zhang, J., Sakamoto, Y., Kiba, T., Yoshida, S., Matsunaga, S., Sakakibara, H., *Shirasu, K.
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 印刷中 Issue: 20 Pages: 5283-5288
  • DOI: 10.1073/pnas.1619078114
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Small Organ Size 1 and Small Organ Size 2/Dwarf and Low Tillering form a Complex to Integrate Auxin and Brassinosteroid Signaling in Rice. (2017)
  • Author(s): Hirano, K., Yoshida, H., Aya, K., Kawamura, M., Hayashi, M., Hobo, T., Sato-Izawa, K., Kitano, H., Ueguchi-Tanaka, M., Matsuoka, M.
  • Journal Title: Molecular plant Volume: 10 Issue: 2 Pages: 590-604
  • DOI: 10.1104/pp.17.00301
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Transcriptomic and Metabolomic Reprogramming from Roots to Haustoria in the Parasitic Plant, Thesium chinense. (2017)
  • Author(s): Ichihashi Y, Kusano M, Kobayashi M, Suetsugu K, Yoshida S, Wakatake T, Kumaishi K, Shibata A, Saito K, Shirasu K.
  • Journal Title: Plant Cell Physiology Volume: 59 Issue: 4 Pages: 724-733
  • DOI: 10.1093/pcp/pcx200
  • NAID: 120006488507
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Local auxin biosynthesis mediated by a YUCCA flavin monooxygenase regulates haustorium development in the parasitic plant Phtheirospermum japonicum. (2016)
  • Author(s): Ishida, J.K., Wakatake, T., Yoshida, S., Takebayashi, Y., Kasahara, H., Wafula, E., dePamphilis, C. W., Namba, S., Shirasu K.
  • Journal Title: Plant Cell Volume: 28 Issue: 8 Pages: 1795-1814
  • DOI: 10.1105/tpc.16.00310
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Haustorial hairs are specialized root hairs that support parasitism in the facultative parasitic plant, Phtheirospermum japonicum (2016)
  • Author(s): Songkui Cui, Takanori Wakatake, Kei Hashimoto, Simon Saucet, Kiminori Toyooka, Satoko Yoshida and Ken Shirasu
  • Journal Title: Plant Physiology Volume: 170 Issue: 3 Pages: 1492-1503
  • DOI: 10.1104/pp.15.01786
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Haustorium, a Specialized Invasive Organ in Parasitic Plants. (2016)
  • Author(s): Yoshida, S., Cui, S., Ichihashi, Y., Shirasu, K.
  • Journal Title: Ann. Rev. Plant Biol. Volume: 67 Issue: 1 Pages: 643-667
  • DOI: 10.1146/annurev-arplant-043015-111702
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 根寄生植物の寄生メカニズム (2016)
  • Author(s): 若竹崇雅、吉田聡子、白須賢
  • Journal Title: 生物の科学 遺伝 Volume: 70 Pages: 289-293
• [Journal Article] 寄生植物コシオガマの吸器形成機構 (2016)
  • Author(s): 若竹崇雅、吉田聡子、白須賢
  • Journal Title: BSJ-Review Volume: 7 Pages: 241-249
• [Journal Article] Convergent evolution of strigolactone perception enabled host detection in parasitic plants. (2015)
  • Author(s): Conn, C. E., Bythell-Douglas, R., Neumann, D., Yoshida, S., Whittington, B., Westwood, J. H., Shirasu, K., Bond, C. S., Dyer, K. A. Nelson, D. C.
  • Journal Title: Science Volume: 349 Issue: 6247 Pages: 540-543
  • DOI: 10.1126/science.aab1140
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The WRKY45-dependent signaling pathway is required for resistance against Striga parasitism (2015)
  • Author(s): Mutuku JM, Yoshida S, Shimizu T, Ichihashi Y, Wakatake T, Seo M, Takahashi A, Shirasu K
  • Journal Title: Plant Physiology Volume: 168 Issue: 3 Pages: 1152-1163
  • DOI: 10.1104/pp.114.256404
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Apoplastic interactions between plants and plant root intruders (2015)
  • Author(s): Mitsumasu K, Seto Y, Yoshida S.
  • Journal Title: Frontiers in Plant Science Volume: 6 Pages: 617-617
  • DOI: 10.3389/fpls.2015.00617
  • Peer Reviewed / Open Access
• [Journal Article] Transcriptomics exposes the uniqueness of parasitic plants. (2015)
  • Author(s): Ichihashi, Y., Mutuku, J.M., Yoshida, S., and Shirasu, K.
  • Journal Title: Briefings in Functional Genomics Volume: in press Issue: 4 Pages: 0-0
  • DOI: 10.1093/bfgp/elv001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 遺伝子から見た根寄生植物の生存戦略 (2014)
  • Author(s): 吉田聡子、若竹崇雅、白須賢
  • Journal Title: 植物の生長調節 Volume: 49 Pages: 66-73
  • NAID: 110009815847
  • Acknowledgement Compliant
• [Presentation] Genome structure and gene transfer in parasitic plants (2017)
  • Author(s): Satoko Yoshida and Ken Shirasu
  • Organizer: 日本植物生理学会第５８回年会
  • Place of Presentation: 鹿児島大学 郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
  • Invited
• [Presentation] モデル寄生植物コシオガマの変異体原因遺伝子同定 (2017)
  • Author(s): Songkui Cui、 重信秀治、西山智明、柴田朋子、長谷部光泰、白須賢、吉田聡子
  • Organizer: 日本植物学会
• [Presentation] Host lignin composition affects haustorium induction in parasitic plants (2017)
  • Author(s): Songkui Cui, Syogo Wada, Yuki Tobimatsu, Yuri Takeda, Toshiyuki Takano, Toshiaki Umezawa, Ken Shirasu and Satoko Yoshida
  • Organizer: 日本植物生理学会
• [Presentation] 寄生植物ストライガの吸器誘導における活性酸素種の役割 (2017)
  • Author(s): Syogo Wada, Songkui Cui and Sotoko Yoshida
  • Organizer: 日本植物生理学会
• [Presentation] Ethylene signaling controls haustorium development in parasitic plant Phtheirospermum japonicum (2017)
  • Author(s): Songkui Cui, Ken Shirasu , Satoko Yoshida
  • Organizer: 日本植物生理学会
• [Presentation] Genomes and genetics of parasitic plants in Orobanchaceae (2017)
  • Author(s): Satoko Yoshida
  • Organizer: The 14th World Congress on Parasitic Plants
  • Int'l Joint Research / Invited
• [Presentation] 寄生植物コシオガマにおけるsubtilaseの分類と発現動態解析 (2017)
  • Author(s): 小川哲史、若竹崇雅、吉田聡子、市橋泰範、白須賢
  • Organizer: 日本植物生理学会
• [Presentation] A yeast-one hybrid assay to explore transcription factors that initiate haustorium development in parasitic plants (2017)
  • Author(s): Takanori Wakatake, Satoko Yoshida, Ken Shirasu
  • Organizer: 日本植物生理学会
• [Presentation] ハマウツボ科寄生植物の寄生器官の発生と形づくり (2017)
  • Author(s): 吉田聡子
  • Organizer: 日本農芸化学会第177回中部支部会シンポジウム
  • Invited
• [Presentation] 植物に寄生する植物：寄生植物の宿主の見つけ方 (2017)
  • Author(s): 吉田聡子
  • Organizer: 日本分子生物学会
  • Invited
• [Presentation] Genomics and genetics in parasitic Orobanchaceae (2016)
  • Author(s): Satoko Yoshida
  • Organizer: 奈良先端大異分野融合ワークショップFrontiers in parasitic plant and host interactions
  • Place of Presentation: 奈良先端大(奈良県生駒市）
  • Year and Date: 2016-08-30
• [Presentation] 寄生植物ストライガのゲノムにおける植物免疫関連遺伝子の解析 (2016)
  • Author(s): 吉田聡子, Thomas Spallek, Anuphon Laohavisit, Juliane K. Ishida,白須賢
  • Organizer: 日本植物病理学会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] Intimate plant-plant interaction between parasitic plants and their hosts (2016)
  • Author(s): Satoko Yoshida, Songkui Cui, Takanori Wakatake, Thomas Spallek, Yasunori Ichihashi, Simon Saucet, Ken Shirasu
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
  • Invited
• [Presentation] ハマウツボ科寄生植物の寄生形質獲得と適応進化 (2015)
  • Author(s): 吉田聡子、Thomas Spallek, 市橋泰範、Songkui Cui, 若竹崇雅、Juliane Ishida, 白須賢
  • Organizer: 日本植物学会
  • Place of Presentation: 新潟 朱鷺ホール
  • Year and Date: 2015-09-06
  • Invited
• [Presentation] Genomic and genetic analyses of haustorium formation using Phtheirospermum japonicum as a model parasitic plant. (2015)
  • Author(s): Satoko Yoshida, Takanori Wakatake, Songkui Cui, Juliane K. Ishida, Thomas Spallek, Simon Saucet, and Ken Shirasu
  • Organizer: The 13th World Congress on Parasitic Plants
  • Place of Presentation: 昆明、中国
  • Year and Date: 2015-07-05
  • Int'l Joint Research
• [Presentation] 寄生植物ストライガの感染戦略と宿主植物の応答 (2015)
  • Author(s): 吉田聡子
  • Organizer: シンポジウム｢寄生・共生インシデント｣
  • Place of Presentation: 東京大学
  • Year and Date: 2015-04-28
  • Invited
• [Presentation] 寄生植物ストライガの抵抗性におけるジャスモン酸とサリチル酸シグナル経路の役割 (2015)
  • Author(s): Musembi Mutuku、吉田聡子、清水崇史、市橋泰範、高橋章、瀬尾光範、白須賢
  • Organizer: 日本植物病理学会
  • Place of Presentation: 明治大学
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] 寄生植物と宿主植物の細胞壁をめぐる攻防 (2015)
  • Author(s): 吉田聡子, Musembi Mutuku, Songkui Cui, 堀千明, 清水崇, 市橋安範, 出村拓, 白須賢
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] Molecular and genomic analyses of the witchweed Striga (2014)
  • Author(s): Satoko Yoshida
  • Organizer: 筑波大学 Global Science week
  • Place of Presentation: 筑波大学
  • Year and Date: 2014-09-29
  • Invited
• [Presentation] 寄生植物ストライガの全ゲノム解読 (2014)
  • Author(s): 吉田聡子, Thomas Spallek, Songkui Cui, 市橋 泰範, Seungill Kim, Yong-Min Kim, 真鍋 理一郎, Michael Timko, Doil Choi, 白須 賢
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学生田キャンパス
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] ゲノム・トランスクリプトーム解析からみた植物の寄生 (2014)
  • Author(s): 吉田聡子
  • Organizer: 第８回細菌学若手コロッセウム
  • Place of Presentation: ニセコ
  • Year and Date: 2014-08-07
  • Invited
• [Presentation] Plant-plant communications in rhizosphere: interactions and signaling between root parasites and their host plants (2014)
  • Author(s): Satoko Yoshida, Juliane K. Ishida, Takanori Wakatake, Songkui Cui, Ken Shirasu
  • Organizer: 第５５回日本植物生理学会年会
  • Place of Presentation: 富山大学
  • Invited
• [Presentation] Genome and transcriptome analyses of Striga spp. (2013)
  • Author(s): Satoko Yoshida, Ri-ichiroh Manabe, Seungil Kim, Thomas Spallek, Musembi Mutuku, Michael Timko, Doil Choi, Ken Shirasu
  • Organizer: 12th World Congress on Parasitic Plants
  • Place of Presentation: Sheffield, UK
• [Patent(Industrial Property Rights)] 吸器形成阻害剤 (2018)
  • Inventor(s): 和田将吾、Songkui Cui, 吉田聡子
  • Industrial Property Rights Holder: 和田将吾、Songkui Cui, 吉田聡子
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-010715
  • Filing Date: 2018