| Budget Amount *help |
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Outline of Final Research Achievements |
Recent studies have shown glycolysis acts central role for regulation of cellular character in cancer and stem cells. Although these studies strongly suggest that glycolysis has an important role in animal development, role of glycolysis in the developmental processes, such as germline development, has remained elusive.
For revealing metabolic status of germline, we performed metabolomics of primordial germ cells (PGCs) of Drosophila. We found that glycolysis is enhanced in PGCs in early embryos. By investigating the role of glycolysis in PGCs by RNAi, we found glycolysis regulates induction of PGC cell death during embryogenesis. In addition, through developing quantitative imaging analysis for mitochondria, we found that amount of mitochondria was significantly reduced in PGCs causing cell death. Our study strongly suggested that glycolysis dependent PGC cell death has important role to eliminate the PGCs with unwanted mitochondria.
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