In vitro analysis of functions of cohesin in replication-coupled sister-chromatid cohesion and DNA double-strand break repair
| Project/Area Number |
25711022
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Kyushu University (2016)
Osaka University (2013-2015) |
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Principal Investigator |
Takahashi Tatsuro 九州大学, 理学(系)研究科(研究院), 准教授 (50452420)
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| Research Collaborator |
HIGASHI Torahiko
KAWASOE Yoshitaka
YAMAGUCHI Yohko
TERUI Riki
HAYASHI Sae
KAYUKAWA Taiki
ODA Satomi
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | DNA複製 / 染色体接着 / 相同組換え / 二重鎖切断 / ツメガエル卵抽出液 / コヒーシン / アセチル化 / 姉妹染色体接着 / DNA二重鎖切断 / DNA損傷修復 / ツメガエル / 染色体分配 / DNA修復 / 無細胞系 |
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| Outline of Final Research Achievements |
Sister-chromatid cohesion, mediated by the cohesin complex, facilitates precise segregation of sister chromosomes at M-phase and promotes repair of DNA double-strand breaks (DSBs). However, how cohesin promotes those reactions has not been clearly understood. Using Xenopus egg extracts and biochemical reconstitution with purified proteins as model systems, we revealed functional relationship between XEco2 cohesin acetyltransferase, a key enzyme in the cohesion establishment reaction, and multiple replication proteins. We also constructed in vitro model systems for post-replicative DSB repair, and analyzed involvement of cohesin in the repair reaction. This study clarified novel functional interaction between sister-chromatid cohesion and DNA replication.
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Report
(5 results)
Research Products
(37 results)
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[Journal Article] MutS stimulates the endonuclease activity of MutL in an ATP-hydrolysis-dependent manner.2013
Author(s)
Shimada, A., Kawasoe, Y., Hata, Y., Takahashi, T.S., Masui, R., Kuramitsu, S., Fukui, K.
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Journal Title
FEBS Journal
Volume: 280 (14)
Issue: 14
Pages: 3467-3479
DOI
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Peer Reviewed
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[Presentation] MutSα-dependent eviction of nucleosomes around mismatch sites in Xenopus egg extracts2014
Author(s)
Tatsuro Takahashi, Riki Terui, Kanae Taki, Koji Nagao, Takuro Nakagawa, Uimiko Kubota, Haruhiko Takisawa, Chikashi Obuse, Hisao Masukata
Organizer
International Conference: Replication, repair, and transcription; coupling mechanisms and chromatin dynamics for genome integrity
Place of Presentation
京都市左京区 京都大学 時計台100周年記念ホール
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