| Budget Amount *help |
¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2013: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
Sister-chromatid cohesion, mediated by the cohesin complex, facilitates precise segregation of sister chromosomes at M-phase and promotes repair of DNA double-strand breaks (DSBs). However, how cohesin promotes those reactions has not been clearly understood. Using Xenopus egg extracts and biochemical reconstitution with purified proteins as model systems, we revealed functional relationship between XEco2 cohesin acetyltransferase, a key enzyme in the cohesion establishment reaction, and multiple replication proteins. We also constructed in vitro model systems for post-replicative DSB repair, and analyzed involvement of cohesin in the repair reaction. This study clarified novel functional interaction between sister-chromatid cohesion and DNA replication.
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