Is there aneuploid evolution? (II) Comparative genome analysis and verification by production of alien chromosome addition line

• Project/Area Number: 25711024
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Chiba University
• Principal Investigator: KIKUCHI SHINJI 千葉大学, 大学院園芸学研究科, 助教 (80457168)
• Project Period (FY): 2013-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥27,430,000 (Direct Cost: ¥21,100,000、Indirect Cost: ¥6,330,000); Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
• Keywords: 異数体 / トレニア / 染色体 / 種分化 / FISH解析 / 遺伝資源 / 核型 / ゲノム分析 / 異数性 / ゲノム / 比較ゲノム解析

Outline of Final Research Achievements

During meiosis in interspecific hybrids of T. fournieri (2n=2x=18) x T. baillonii (2n=2x=16), eight bivalents by interspecific pairing and a univalent of T. fournieri chromosome were observed, Phylogenetic analysis suggests a hypothesis that T. baillonii lost the chromosome during the speciation. In order to verily the hypothesis, comparative genome analysis of T. fournieri and T. baillonii was conducted. The results indicated that T. baillonii genomic DNAs can find on all nine of T. fournieri chromosomes and the loss of a chromosome pair might be not true during the speciation.

Research Products

• [Int'l Joint Research] カセサート大学(タイ)
• [Journal Article] High genomic affinity between Torenia baillonii and Torenia fournieri revealed by genome analysis using a triploid hybrid. (2017)
  • Author(s): Buntarika NUNTHA, Shinj KIKUCHI, Thuya TAYCHASINPITAK, Hidenori SASSA, Takato KOBA
  • Journal Title: Cytologia Volume: 82(2)
  • NAID: 130006769799
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] New karyotypes of an interspecific hybrid of Torenia fournieri and Torenia baillonii and its progenies. (2016)
  • Author(s): Buntarika NUNTHA, Shinji KIKUCHI, Thunya TAYCHASINPITAK, Hidenori SASSA, Takato KOBA
  • Journal Title: Chromosome Science Volume: 19
  • NAID: 130006837149
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mutation breeding of Thai native torenia (Torenia fournieri Lind.) by gamma-ray irradiation. (2016)
  • Author(s): Thunya TAYCHASINPITAK, Shinji KIKUCHI, Anchalee JALA, Theerachai THANANANTA, Nattapong CHANCHULA
  • Journal Title: Thai Journal of Science and Technology Volume: 5 Pages: 190-199
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chromosome number increasing in Torenia hybrid by application of colchicine tablet. (2016)
  • Author(s): Lucia RAGASOVA, Ivo ONDRASEK, Thunya TAYCHASINPITAK, Suthasinee PINTHONG, Banthita PENSURIYA, Valerie Web SUWANSEREE, Shinji KIKUCHI
  • Journal Title: Thai Journal of Science and Technology Volume: 5 Pages: 200-212
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Radiosensitivity of in vitro cultured Torenia fournieri Lind. from Thailand by γ-ray irradiation. (2015)
  • Author(s): Nattapong Chanchula, Thunya Taychasinpitak, Anchalee Jala, Theerachai Thanananta, Shinji Kikuchi
  • Journal Title: International Transaction Journal of Engineering, Management, & Applied Science & Technologies. Volume: 6 Pages: 157-164
  • DOI: 10.14456/itjemast.2015.8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Induction of somatic embryogenesis in Torenia fournieri Lind. (2015)
  • Author(s): Nattapong Chanchula, Thunya Taychasinpitak, Anchalee Jala, Theerachai Thanananta, Shinji Kikuchi.
  • Journal Title: International Transaction Journal of Engineering, Management, & Applied Science & Technologies. Volume: 6 Pages: 165-171
  • DOI: 10.14456/itjemast.2015.7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effect of gout drug treatments on survival rate and morphological change of Lindernia sp. in vitro. (2015)
  • Author(s): Kamontip Sungkaew, Thunya Taychasinpitak, Shermarl Wongchaochant, Patana Sukprasert, Shinji Kikuchi
  • Journal Title: International Transaction Journal of Engineering, Management, & Applied Science & Technologies. Volume: 6 Pages: 191-201
  • DOI: 10.14456/itjemast.2015.5
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Seed formation in triploid loquat (<i>Eriobotrya japonica</i>) through cross-hybridization with pollen of diploid cultivars (2014)
  • Author(s): Kikuchi, S., Iwasuna, M., Kobori, A., Tsutaki, Y., Yoshida, A., Murota, Y., Nishino, E., Sassa, H. and Koba, T.
  • Journal Title: Breeding Science Volume: 64 Issue: 2 Pages: 176-182
  • DOI: 10.1270/jsbbs.64.176
  • NAID: 130004146436
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Presentation] トレニアの種間雑種における染色体行動の解析 (2016)
  • Author(s): 菊池真司
  • Organizer: 第67回 染色体学会 年会
  • Place of Presentation: 東京大学（東京都、文京区）
  • Invited
• [Presentation] Genomic affinity level between Torenia baillonii and Torenia fournieri measured by genome analysis with a triploid hybrid (2016)
  • Author(s): Buntarika NUNTHA, Shinji KIKUCHI, Hidenori SASSA, Takato KOBA
  • Organizer: 第67回 染色体学会 年会
  • Place of Presentation: 東京大学（東京都、文京区）
• [Presentation] Application of detached-leaf technique and gout drug treatment to induced tetraploid in Torenia hybrida (T. fournieri x T. asiatica) (2015)
  • Author(s): Chanchula, N., Taychasinpitak, T., Jala, A., Thanananta, T., Kikuchi, S.
  • Organizer: The 5th Asian Chromosome Colloquium
  • Place of Presentation: カセサート大学（バンコク, タイ）
  • Year and Date: 2015-04-29
  • Int'l Joint Research
• [Presentation] Torenia fournieriにおける各染色体の識別及び核型解析 (2014)
  • Author(s): 小澤翔太、菊池真司、鈴木孝征、豊田敦、藤山秋佐夫、東山哲也、佐々英徳、木庭卓人
  • Organizer: 染色体学会 第65回年会
  • Place of Presentation: 岡山県倉敷市芸文館
  • Year and Date: 2014-10-24 – 2014-10-25
• [Presentation] 野生アズキ・東日本集団の相互転座と氷河期レフュジアからの分布変遷 (2014)
  • Author(s): Wang Lixia、 菊池真司、武藤千秋、内藤健、伊勢村武久、石本政男、Xuzhen Cheng、加賀秋人、友岡憲彦
  • Organizer: 染色体学会 第65回年会
  • Place of Presentation: 岡山県倉敷市芸文館
  • Year and Date: 2014-10-24 – 2014-10-25
• [Presentation] Comparative karyotype analysis in cucmber, Cucumis sativus, by fluorescence in situ hybridization technique (2014)
  • Author(s): Nguyen Anh Duc, Shinji Kikuchi, Hidenori Sassa, Takato Koba
  • Organizer: 染色体学会 第65回年会
  • Place of Presentation: 岡山県倉敷市芸文館
  • Year and Date: 2014-10-24 – 2014-10-25