Analysis of embryo facing endosperm region regulating embryo-endosperm ratio of albuminous seed in rice

• Project/Area Number: 25712001
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: The University of Tokyo
• Principal Investigator: Hibara Ken-ichiro 東京大学, 農学生命科学研究科, 助教 (10595713)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000); Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2014: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000); Fiscal Year 2013: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: イネ / 胚発生 / 胚乳発生 / 細胞死 / 胚サイズ / 有胚乳種子

Outline of Final Research Achievements

I have been previously found that the embryo facing endosperm region (EFR) is important for proper embryo-endosperm ratio of albuminous seed in rice. This study investigated the molecular mechanisms for developmental process of EFR, which was divided into three developmental phases (initiation, maintenance and regional determination). I performed the cytological characterization of EFR in 2-4 days after pollination, the cloning of EML1 gene involved in the initiation phase, RE3 gene involved in the maintenance phase and HER gene act downstream of GE gene to influence the regional determination. I inferred from these results that the oxygen sensing and auxin signaling molecules might be involved in the organization of EFR in rice.

Research Products

• [Journal Article] A reduction of sucrose phosphate synthase (SPS) activity affects sucrose/starch ratio in leaves but does not inhibit normal plant growth in rice (2016)
  • Author(s): Yoichi Hashida, Tatsuro Hirose, Masaki Okamura, Ken-ichiro Hibara, Ryu Ohsugi, Naohiro Aoki
  • Journal Title: Plant Science Volume: 253 Pages: 40-49
  • DOI: 10.1016/j.plantsci.2016.08.017
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Jasmonate regulates juvenile-adult phase transition in rice. (2016)
  • Author(s): Hibara, K., Isono, M., Mimura, M., Sentoku, N., Kojima, M., Sakakibara, H., Kitomi, Y., Yoshikawa, T., Itoh, J., and Nagato, Y.
  • Journal Title: Development Volume: 143 Pages: 3407-3416
  • DOI: 10.1242/dev.138602
  • Peer Reviewed / Open Access
• [Journal Article] Genome-wide prediction of spatio-temporal gene expression patterns during early embryogenesis in rice. (2016)
  • Author(s): Jun-ichi Itoh, Yutaka Sato, Yutaka Sato, Ken-ichiro Hibara, Sae Shimizu-Sato, Hiromi Kobayashi, Hinako Takehisa, Karen Sanguinet, Nobukazu Namiki, Yoshiaki Nagamura
  • Journal Title: Development Volume: 143 Pages: 1217-1227
  • DOI: 10.1242/dev.123661
  • Peer Reviewed
• [Journal Article] Barley NARROW LEAFED DWARF1 encoding a WUSCHEL-RELATED HOMEOBOX 3 (WOX3) regulates the marginal development of lateral organs. (2016)
  • Author(s): Yoshikawa, T.,Tanaka, S-Y., Masumoto, Y., Nobori, N., Ishii, Hibara K-I, Itoh J-I, Tanisaka T. and Taketa S.
  • Journal Title: Breeding Science Volume: 印刷中
  • NAID: 130005160889
  • Peer Reviewed
• [Journal Article] Molecular genetics of the events of early differentiation in rice (2015)
  • Author(s): 桧原健一郎
  • Journal Title: 育種学研究 Volume: 17(3) Pages: 115-119
  • NAID: 130005096829
• [Journal Article] The rice FISH BONE gene encodes a tryptophan aminotransferase, which affects pleiotropic auxin-related processes. (2014)
  • Author(s): Yoshikawa, T., Ito, M., Sumikura, T., Nakayama, A., Nishimura, T., Kitano, H., Yamaguchi, I., Koshiba T., Hibara KI., Nagato Y. and Itoh JI.
  • Journal Title: The Plant Journal Volume: 未定
  • Peer Reviewed
• [Journal Article] Organ fusion and defective shoot development in oni3 mutants of rice (2014)
  • Author(s): Akiba T, Hibara K, Kimura F, Tsuda K, Shibata K, Ishibashi M, Moriya C, Nakagawa K, Kurata N, Itoh J-I, Ito Y
  • Journal Title: Plant Cell Physiol Volume: 55 Issue: 1 Pages: 42-51
  • DOI: 10.1093/pcp/pct154
  • NAID: 40019968717
  • Peer Reviewed
• [Journal Article] 種子形成シナリオの分子遺伝学(Molecular genetics of seed formation scenario). (2013)
  • Author(s): 伊藤純一、佐藤豊、桧原健一郎、服部束穂、岩崎行玄
  • Journal Title: 育種学研究 Volume: 15 Pages: 158-133
• [Journal Article] GIANT EMBRYO, encoding CYP78A13, affects size balance between embryo and endosperm in rice. (2013)
  • Author(s): Nagasawa, N., Hibara, K., Heppard E.P., Vander Velden., K.A., Luck, S., Beatty M., Nagato, Y. and Sakai H.
  • Journal Title: The Plant Journal Volume: 未定 Issue: 4 Pages: 592-605
  • DOI: 10.1111/tpj.12223
  • Peer Reviewed
• [Journal Article] ABNORMAL SHOOT IN YOUTH, a Homolog of Molybdate Transporter Gene, Regulates Early Shoot Development in Rice (2013)
  • Author(s): Hibara, K., Hosoki, W., Hakoyama, T., Ohmori,Y., Fujiwara, T., Itoh, J and Nagato, Y.
  • Journal Title: American Journal of Plant Sciences Volume: 4 Issue: 05 Pages: 1-9
  • DOI: 10.4236/ajps.2013.45a001
  • Peer Reviewed / Open Access
• [Presentation] 胚と胚乳サイズを規定する分子機構の解明に向けて (2016)
  • Author(s): 桧原 健一郎
  • Organizer: 第37回種子生理生化学会
  • Place of Presentation: 兵庫県南あわじ市
  • Year and Date: 2016-11-11
• [Presentation] 胚/胚乳比率を制御する分子機構の解析 (2016)
  • Author(s): 桧原 健一郎
  • Organizer: イネ遺伝学・分子生物学ワークショップ2016
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2016-07-04
• [Presentation] 器官や果実サイズを制御するCYP78の分子遺伝学的解析 (2016)
  • Author(s): 水野泉、伊藤純一、桧原健一郎
  • Organizer: 育種学会第129回講演会
  • Place of Presentation: 横浜市立大学
  • Year and Date: 2016-03-21
• [Presentation] A framework for identification of the substrate and signaling pathway of CYP78 regulating lateral organ and seed size. (2016)
  • Author(s): Izumi Mizuno, Jun-ichi Itoh, Ken-ichiro Hibara
  • Organizer: 第57回植物生理学会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] heraclesは巨大胚変異体(ge)を半優性に抑圧する (2015)
  • Author(s): 水野泉、伊藤純一、桧原健一郎
  • Organizer: 育種学会第128回講演会
  • Place of Presentation: 新潟大学
  • Year and Date: 2015-09-12
• [Presentation] イネにおける初期発生制御機構の分子遺伝学的研究 (2015)
  • Author(s): 桧原 健一郎
  • Organizer: 育種学会第127回講演会
  • Place of Presentation: 東京都町田市
  • Year and Date: 2015-03-21
• [Presentation] イネ胚乳で発現するREDUCED EMBRYO1(RE1),RE2は胚-胚乳比率を制御する (2015)
  • Author(s): 小林裕美, 永澤信洋, 佐藤豊, 伊藤純一, Sakai Hajime, 長戸康郎, 桧原健一郎
  • Organizer: 育種学会第127回講演会
  • Place of Presentation: 東京都町田市
  • Year and Date: 2015-03-21
• [Presentation] 頂端分裂組織の形態に異常を生じるイネ突然変異体camelの表現型解析と遺伝子同定 (2014)
  • Author(s): 小林 久美子, 桧原 健一郎, 伊藤 純一, 長戸 康郎
  • Organizer: 育種学会第126回講演会
  • Place of Presentation: 宮崎県都城市
  • Year and Date: 2014-09-26
• [Presentation] 葉や胚のサイズを制御するCYP78 の基質同定を目指して (2014)
  • Author(s): 水野 泉, 三村 真生, 伊藤 純一, 桧原 健一郎
  • Organizer: 育種学会第126回講演会
  • Place of Presentation: 宮崎県都城市
  • Year and Date: 2014-09-26
• [Presentation] 地上部に多面的な異常を示すイネmkb3 変異体の解析 (2014)
  • Author(s): 島野 里美，桧原 健一郎，伊藤 純一
  • Organizer: 育種学会第126回講演会
  • Place of Presentation: 宮崎県都城市
  • Year and Date: 2014-09-26
• [Presentation] イネ種子の胚－胚乳比率を規定する胚周辺胚乳領域の解析 (2014)
  • Author(s): 桧原健一郎、小林裕美、伊藤純一、長戸康郎
  • Organizer: 第55回植物生理学会
  • Place of Presentation: 富山大学
• [Presentation] 胚ー胚乳比率に影響を及ぼすEMBRYOLESS1遺伝子の解析 (2013)
  • Author(s): 堀島佑月、琴梨世、石川亮、桧原健一郎、伊藤純一、長戸康郎
  • Organizer: 育種学会第124回講演会
  • Place of Presentation: 鹿児島大学
• [Presentation] イネ胚の巨大化を促す遺伝学的要因 (2013)
  • Author(s): 桧原健一郎、小林裕美、伊藤純一、長戸康郎
  • Organizer: 育種学会第124回講演会
  • Place of Presentation: 鹿児島大学
• [Presentation] Identification of ADAXIALIZED LEAF2 gene involved in the patterning of leaf, embryo and endosperm throughout proper epidermal development (2013)
  • Author(s): Hibara, K., Itoh, J. and Nagato Y.
  • Organizer: The 7th International Rice Genetics Symposium
  • Place of Presentation: Manila, Philipines
• [Remarks] 東京大学農学部・農学生命科学研究科 生産・環境生物学専攻 育種学研究室
  • URL: http://papilio.ab.a.u-tokyo.ac.jp/pbg/pages%28html.%29/rooms%20of%20LABO.html