Elucidation of mechanism for esterified carotenoids in tomato petal

• Project/Area Number: 25712004
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Horticultural science
• Research Institution: University of Tsukuba
• Principal Investigator: Ariizumi Tohru 筑波大学, 生命環境系, 准教授 (70575381)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: トマト / カロテノイド / 変異体 / 脂肪酸 / プラストグロブリ / 園芸学 / 育種学 / 遺伝子 / 遺伝学 / 酵素 / 植物

Outline of Final Research Achievements

This research aimed to understand molecular mechanism for esterified carotenoid using tomato mutants named pale yellow petal 1 and pyp2 that produce petals with reduced pigmentation due to reduced accumulation of the esterified carotenoid. We identified candidate genes for pyp1 and pyp2 mutants and performed complementation experiments, demonstrating that both genes were the responsible. Biochemical analyses showed that PYP1 most likely catalyzes esterification of xanthophylls with mid-chain fatty acids such as C14:0 and C16:0 as substrate. Further, plastglobule formation in plastid is highly associated with accumulation of esterified xanthophylls. Moreover, PYP1 is localized near the plastoglobule formation. These results indicated that esterified formation is essential for plastoglobule formation.

Research Products

• [Int'l Joint Research] フランス国立農業研究所（INRA）(フランス)
• [Journal Article] Identification of the Carotenoid Modifying Gene PALE YELLOW PETAL 1 as an Essential Factor in Xanthophyll Esterification and Yellow Flower Pigmentation in Tomato (Solanum lycopersicum). (2014)
  • Author(s): Ariizumi T, Kishimoto S, Kakami R, Maoka T, Hirakawa H, Suzuki Y, Ozeki Y, Shirasawa K, Bernillon S, Okabe Y, Moing A, Asamizu E, Rothan C, Ohmiya A & Ezura H
  • Journal Title: The Plant Journal Volume: 79 Issue: 3 Pages: 453-465
  • DOI: 10.1111/tpj.12570
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] PYP2 mutation disturbs normal yellow pigmentation of flower in tomato (Solanum lycopersicum) (2016)
  • Author(s): Ariizumi T, Takezawa S, Kishimoto S, Hirasawa H, Shirasawa S, Omiya A, Bertillon S, Moing A, Rothan C, Okabe Y, Ezura H
  • Organizer: The 13th Japan Solanaceae Consortium JSOL2016
  • Place of Presentation: 国際基督教大学（東京・三鷹市）
  • Year and Date: 2016-11-25
  • Int'l Joint Research
• [Presentation] Enhancement of tomato qulity by the breeding tequniques. (2016)
  • Author(s): Ariizumi T
  • Organizer: Tsukuba-France Joint Conference
  • Place of Presentation: 筑波大学、茨城県つくば市
  • Year and Date: 2016-01-22
  • Invited
• [Presentation] 育種技術の力でトマトを高品質化する (2016)
  • Author(s): 有泉亨・江面浩
  • Organizer: 品種改良ユーザー会
  • Place of Presentation: 理化学研究所、埼玉県和光市
  • Year and Date: 2016-01-21
  • Invited
• [Presentation] 園芸作物トマトの遺伝資源創成と果実発達遺伝子群の同定 (2015)
  • Author(s): 有泉亨
  • Organizer: 東京園芸懇話会
  • Place of Presentation: 筑波大学、茨城県つくば市
  • Year and Date: 2015-12-08
  • Invited
• [Presentation] Biochemical and Ultrastructural Studies of a Novel Tomato Mutant pale yellow petal 2 Revealed that Xanthophyll Accumulation are Important for Normal Chromoplast Differentiation. (2015)
  • Author(s): Takezawa S, Kishimoto S, Hirakawa H, Shirasawa K, Omiya A, Okabe A,Ezura H and Ariizumi T
  • Organizer: International Symposium on Solanaceae Cenferance 2015 (SOL2015)
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] Towards improved fruit set efficiency using tomato genetic resources. (2015)
  • Author(s): Ariizumi T, Shinozaki Y, Okabe Y, Hoshikawa K, Shikata M, Yano R, Hao S, Takahara M, Takei H, Benard C, Prodhomme D, Gibon Y, Kanayama Y, Kubo Y, Aoki K and Ezura H
  • Organizer: International Symposium on Solanaceae Cenferance 2015 (SOL2015)
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2015-10-05
  • Int'l Joint Research / Invited
• [Presentation] Biochemical Studies of the Novel Tomato Mutant pale yellow petal 2 (2015)
  • Author(s): 竹澤里実、岸本早苗、平川英樹、白澤健太、 大宮あけみ、江面 浩、有泉亨
  • Organizer: 第33回日本植物細胞分子生物学会
  • Place of Presentation: 東京大学、文京区本郷
  • Year and Date: 2015-08-10
• [Presentation] トマトのPALE YELLOW PETAL1遺伝子はエステル化カロテノイドの蓄積と花弁黄色化に必須である。 (2014)
  • Author(s): 有泉亨、岸本早苗、眞岡孝至、平川英樹、白澤健太、岡部佳宏、竹澤里見、大宮あけみ、江面浩
  • Organizer: 第32回 日本植物細胞分子生物学会
  • Place of Presentation: いわて県民情報交流センター（岩手県盛岡市）
  • Year and Date: 2014-08-21 – 2014-08-22
• [Remarks]
  • URL: http://ariizumi.tsukuba-olericulture.org/index.php?id=2
• [Remarks] 蔬菜花卉学研究室HP
  • URL: http://ariizumi.tsukuba-olericulture.org
• [Remarks] 研究者HP
  • URL: http://ariizumi.tsukuba-olericulture.org/