| Budget Amount *help |
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
This research aimed to understand molecular mechanism for esterified carotenoid using tomato mutants named pale yellow petal 1 and pyp2 that produce petals with reduced pigmentation due to reduced accumulation of the esterified carotenoid. We identified candidate genes for pyp1 and pyp2 mutants and performed complementation experiments, demonstrating that both genes were the responsible. Biochemical analyses showed that PYP1 most likely catalyzes esterification of xanthophylls with mid-chain fatty acids such as C14:0 and C16:0 as substrate. Further, plastglobule formation in plastid is highly associated with accumulation of esterified xanthophylls. Moreover, PYP1 is localized near the plastoglobule formation. These results indicated that esterified formation is essential for plastoglobule formation.
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