| Budget Amount *help |
¥22,100,000 (Direct Cost: ¥17,000,000、Indirect Cost: ¥5,100,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2014: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2013: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
|
|---|
| Outline of Final Research Achievements |
Legume plants establish endosymbiotic association with rhizobia and form nitrogen-fixing root nodules to acquire nitrogen nutrition. In order to reveal the molecular mechanisms that control symbiotic nitrogen fixation activity of nodule, We here focused on the legume symbiotic mutant that defective in symbiotic nitrogen fixation and rhizobial protein secretion system as a determinant of symbiotic phenotype of the legume mutant. In this work, we carried out functional analyses on the rhizobial protein secretion system and revealed its protein structure, protein secretion activity and effector protein region that is secreted to extracellular component. By using gene disruption mutants and over-expression of the secretion system, we also revealed the effects of the secretion system to symbiotic nitrogen fixation and functional relationships between the causal gene of host mutant and the protein secretion system.
|
