| Budget Amount *help |
¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000)
Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
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| Outline of Final Research Achievements |
Biochemical and molecular biology analyses with antibiotic-overproducing drug-resistant mutants (rifampicin-, streptomycin-, and erythromycin-resistant mutants) of Streptomyces spp. revealed that a mutation in RNA polymerase, ribosomal protein or ribosomal RNA altered not only the transcription and translation apparatus but also the mechanisms of ATP generation and accuracy of DNA replication, resulting in activation of secondary metabolism, suggesting that such a dramatic alteration of cellular function causes increased production of secondary metabolites in Streptomyces strains. In addition, this study found that ribosome-targeting antibiotics at subinhibitory concentrations potentiate the production of secondary metabolites in Streptomyces strains and indicated that activating these strains by utilizing the dose-response effects of ribosome-targeting antibiotics could be used to effectively induce the production of cryptic secondary metabolites.
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