| Budget Amount *help |
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
This study is toward understanding a functional role of Noppera-bo, the novel protein that is required for steroid hormone biosynthesis. Based on our phenotypic analyses using noppera-bo loss-of function mutants of the fruit fly Drosophila melanogaster and the silkworm Bombyx mori, we have found that noppera-bo plays an indispensable role in regulating the behavior of cholesterol in steroidogenic organs. To investigate which molecules can affect Noppera-bo enzymatic activity, we have developed a high through-put screen system with the recombinant Noppera-bo protein, the fluorescent probe to monitor the enzymatic activity and chemical compound library. We have found that some steroids directly bind to Noppera-bo protein and display significant inhibitory effects on Noppera-bo enzymatic activity. These important results imply that an endogenous substrate for Noppera-bo should be a kind of steroids, including sterols.
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