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Elucidation of alkaloid translocation mechanism in plants and application to metabolic engineering.

Research Project

Project/Area Number 25712012
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Applied biochemistry
Research InstitutionKobe Pharmaceutical University

Principal Investigator

Shitan Nobukazu  神戸薬科大学, 薬学部, 教授 (20547880)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywordsアルカロイド / 輸送 / 植物 / タバコ / ニコチン / MATE / トランスポーター / 転流 / MATE transporter
Outline of Final Research Achievements

Plants produce various metabolites including secondary metabolite, such as alkaloids, to adapt to their environment. Most secondary alkaloids are stored in a particular organelle of a specific organ, however, our knowledge of transport mechanism is limited. In this study, we investigated nicotine translocation mechanism from roots to leaves in Nicotiana tabacum. We identified and characterized some transporters, i.e., JAT1, JAT2, and NUP1. These transporters are related to nicotine transport, biosynthesis, and defense mechanism in tobacco. Application of these new knowledge to metabolic engineering might be useful for high production of valuable compounds in future.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • Research Products

    (23 results)

All 2016 2015 2014 2013 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 6 results) Presentation (13 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] Ghent University(ベルギー)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Ghent University(ベルギー)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Secondary metabolites in plants: transport and self-tolerance mechanisms2016

    • Author(s)
      Shitan N.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 4 Issue: 7 Pages: 1-4

    • DOI

      10.1080/09168451.2016.1151344

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Translocation and accumulation of nicotine via distinct spatio-temporal regulation of nicotine transporters in Nicotiana tabacum2015

    • Author(s)
      Shitan N., Hayashida M., Yazaki K.
    • Journal Title

      Plant Signaling and Behavior

      Volume: 10 (7) Issue: 7 Pages: e1035852-e1035852

    • DOI

      10.1080/15592324.2015.1035852

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Tobacco NUP1 transports both tobacco alkaloids and vitamin B6.2015

    • Author(s)
      Kato K., Shitan N., Shoji T., Hashimoto T.
    • Journal Title

      Phytochemistry

      Volume: 113 Pages: 33-40

    • DOI

      10.1016/j.phytochem.2014.05.011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Involvement of the leaf-specific multidrug and toxic compound extrusion (MATE) transporter Nt-JAT2 in vacuolar sequestration of nicotine in Nicotiana tabacum.2014

    • Author(s)
      Shitan N., Minami S., Morita M., Hayashida M., Ito S., Takanashi K., Omote H., Moriyama Y., Sugiyama A., Goossens A., Moriyasu M., Yazaki K.
    • Journal Title

      PLoS One

      Volume: 9(9) Issue: 9 Pages: e108789-e108789

    • DOI

      10.1371/journal.pone.0108789

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Alkaloid transporters in plants.2014

    • Author(s)
      Shitan N., Kato K., Shoji T.
    • Journal Title

      Plant Biotechnology

      Volume: 31 Pages: 453-463

    • NAID

      130004721176

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The multidrug and toxic compound extrusion (MATE) family in plants2014

    • Author(s)
      Takanashi K., Shitan N., Yazaki K.
    • Journal Title

      Plant Biotechnology

      Volume: 31 Pages: 417-430

    • NAID

      130004704199

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] New insights into the transport mechanisms in plant vacuoles.2013

    • Author(s)
      Shitan N., Yazaki K.
    • Journal Title

      Int Rev Cell Mol Biol

      Volume: 305 Pages: 383-433

    • DOI

      10.1016/b978-0-12-407695-2.00009-3

    • Related Report
      2013 Annual Research Report
  • [Presentation] 植物二次代謝生産における輸送蓄積機構の解明2016

    • Author(s)
      士反 伸和
    • Organizer
      Kobe Plant Science研究会
    • Place of Presentation
      神戸大学
    • Year and Date
      2016-07-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] Nicotine translocation and accumulation via distinct spatio-temporal regulation of nicotine transporters in Nicotiana tabacum2016

    • Author(s)
      Shitan N., Minami S., Morita M., Hayashida M., Tsuyama T., Moriyama Y., K Takabe., Goossens A. Yazaki K.
    • Organizer
      17th International Workshop on Plant Membrane Biology
    • Place of Presentation
      Annapolis, USA
    • Year and Date
      2016-06-06
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] タバコ植物におけるニコチン輸送プリンパーミアーゼの探索と組織発現解析2015

    • Author(s)
      士反 伸和、大谷 彩、脇野 真菜、大谷 綾香理、後藤 弓絵、加藤 啓太
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物二次代謝生産における自己耐性と輸送の分子機構に関する研究2015

    • Author(s)
      士反 伸和
    • Organizer
      日本農芸化学会関西支部 第490回講演会・ミニシンポジウム
    • Place of Presentation
      大阪
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] タバコプリンパーミアーゼ遺伝子の組織発現解析2015

    • Author(s)
      士反 伸和、大谷彩、脇野真菜、加藤啓太
    • Organizer
      第10回トランスポーター研究会年会
    • Place of Presentation
      東京
    • Year and Date
      2015-06-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 葉特異的な発現をするタバコMATE型トランスポーターNt-JAT2の機能解析2015

    • Author(s)
      士反 伸和,南 翔太,森田 匡彦,林田 南帆,伊藤 慎悟, 高梨 功次郎, 杉山 暁史,表 弘志, 森山 芳則, Alain Goossens,守安 正恭,矢崎 一史
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] タバコ植物体におけるプリンパーミアーゼ遺伝子の組織発現解析2015

    • Author(s)
      大谷彩、脇野真菜、加藤啓太、士反伸和
    • Organizer
      日本薬学会 第135年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] シロイヌナズナにおけるPUP型輸送体遺伝子の発現解析2015

    • Author(s)
      脇野真菜、大谷彩、加藤啓太、士反伸和
    • Organizer
      日本薬学会 第135年会
    • Place of Presentation
      神戸
    • Year and Date
      2015-03-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植物二次代謝生産における自己耐性と輸送の分子機構に関する研究2015

    • Author(s)
      士反 伸和
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      東京
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] タバコ植物におけるニコチン転流機構の解明2014

    • Author(s)
      士反 伸和, 南 翔太, 加藤 啓太, 森田 匡彦, 庄司 翼, Alain Goossens, 橋本 隆, 矢崎 一史
    • Organizer
      第20回天然薬物の開発と応用シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2014-11-06
    • Related Report
      2014 Annual Research Report
  • [Presentation] タバコ植物におけるpurine permease like transporterの機能解析

    • Author(s)
      加藤 啓太、庄司 翼、士反 伸和、橋本 隆
    • Organizer
      第55回日本植物生理学会年会
    • Place of Presentation
      富山
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニコチン輸送体Nt-C215の発現を変化させた形質転換植物体・培養細胞の作出と選抜

    • Author(s)
      四方 純子、士反 伸和、宮坂 萌菜、後藤 真紀子、芦田 理沙、林田 南帆、古市 智子、Dirk Inze、Alain Goossens、矢崎 一史、守安 正恭
    • Organizer
      第8回トランスポーター研究会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Presentation] タバコ植物体におけるニコチントランスポーターの発現様式の解明

    • Author(s)
      林田 南帆、士反 伸和、南 翔太、森田 匡彦、伊藤 慎悟、四方 純子、森田 淳子、Alain Goossens、Dirk Inze、矢崎 一史、守安 正恭
    • Organizer
      第8回トランスポーター研究会
    • Place of Presentation
      熊本
    • Related Report
      2013 Annual Research Report
  • [Remarks] 神戸薬科大学 生薬化学研究室

    • URL

      http://www.kobepharma-u.ac.jp/edrs/faculty_member_list/natural_medicinal_chemistry.html

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2013-05-21   Modified: 2019-07-29  

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