Elucidation of alkaloid translocation mechanism in plants and application to metabolic engineering.
| Project/Area Number |
25712012
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Applied biochemistry
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| Research Institution | Kobe Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2014: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2013: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | アルカロイド / 輸送 / 植物 / タバコ / ニコチン / MATE / トランスポーター / 転流 / MATE transporter |
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| Outline of Final Research Achievements |
Plants produce various metabolites including secondary metabolite, such as alkaloids, to adapt to their environment. Most secondary alkaloids are stored in a particular organelle of a specific organ, however, our knowledge of transport mechanism is limited. In this study, we investigated nicotine translocation mechanism from roots to leaves in Nicotiana tabacum. We identified and characterized some transporters, i.e., JAT1, JAT2, and NUP1. These transporters are related to nicotine transport, biosynthesis, and defense mechanism in tobacco. Application of these new knowledge to metabolic engineering might be useful for high production of valuable compounds in future.
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Report
(5 results)
Research Products
(23 results)
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[Journal Article] Involvement of the leaf-specific multidrug and toxic compound extrusion (MATE) transporter Nt-JAT2 in vacuolar sequestration of nicotine in Nicotiana tabacum.2014
Author(s)
Shitan N., Minami S., Morita M., Hayashida M., Ito S., Takanashi K., Omote H., Moriyama Y., Sugiyama A., Goossens A., Moriyasu M., Yazaki K.
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Journal Title
PLoS One
Volume: 9(9)
Issue: 9
Pages: e108789-e108789
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] タバコ植物におけるニコチン転流機構の解明2014
Author(s)
士反 伸和, 南 翔太, 加藤 啓太, 森田 匡彦, 庄司 翼, Alain Goossens, 橋本 隆, 矢崎 一史
Organizer
第２０回天然薬物の開発と応用シンポジウム
Place of Presentation
東京
Year and Date
2014-11-06
Related Report
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