| Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
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| Outline of Final Research Achievements |
The effects of genomic constitution in germ cell on gametogenesis was studied using progeny, which were produced by artificial hybridization using loach derived from two genetically different groups and clonal line. Allodiploid males produced a small amount of haploid sperm and female suggested a fertility because of oocyte growth in the ovary. In allotriploids, males showed infertility due to the defect in meiosis, although females showed fertility as well as the allodiploid female. In the infertile allotriploid male, abnormal pairing in meiotic chromosomes were detected by Fluorescence in situ hybridization. Genomic in situ hybridization revealed genetic differences between chromosomes of the two groups. Transcriptome analysis based on RNA-seq to compare gene expressions between gonads with unreduced or reduced gametogenesis showed different expressing genes associated with cell division and cell cycle etc.
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