Development of symbiotic bacteria producing sponge-derived natural products.

• Project/Area Number: 25712024
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: The University of Tokyo
• Principal Investigator: Takada Kentaro 東京大学, 農学生命科学研究科, 助教 (90455353)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000); Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2013: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 海洋天然物 / 海綿動物 / 共生微生物 / 物質生産

Outline of Final Research Achievements

A lot of secondary metabolites with diagnostic structural features and biological activity have been isolated from marine sponges. These metabolites are expected to be produced by symbiotic bacteria in the sponges, however there is few study to identify the bacteria producing sponge-derived natural products. This study demonstrated the identification of the producer of several marine natural products under cultivation-free methods.

Research Products

• [Journal Article] Nazumazoles A-C, Cyclic Pentapeptides Dimerized through a Disulfide Bond from the Marine Sponge Theonella swinhoei. (2015)
  • Author(s): Fukuhara, K.; Takada, K.; Okada, S.; Matsunaga, S.
  • Journal Title: Org. Lett. Volume: 17 Issue: 11 Pages: 2646-2648
  • DOI: 10.1021/acs.orglett.5b01020
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Two Cell Differentiation Inducing Pyridoacridines from a Marine Sponge Biemna sp. and Their Chemical Conversions (2015)
  • Author(s): Moran, D.A.P., Takada, K., Ise, Y., Bontemps, N., Rohan, D., Furihata, K., Okada, S. and Matsunaga, S.
  • Journal Title: Tetrahedron Volume: 71 Issue: 30 Pages: 5013-5018
  • DOI: 10.1016/j.tet.2015.05.070
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cytotoxic linear acetylenes from a marine sponge Pleroma sp. (2015)
  • Author(s): Takahashi, E., Takada, K., Hashimoto, M., Itoh, Y., Ise, Y., Otsuka, S., Okada, S. and Matsunaga, S.
  • Journal Title: Tetrahedron Volume: 71 Issue: 51 Pages: 9564-9570
  • DOI: 10.1016/j.tet.2015.10.062
  • Peer Reviewed
• [Journal Article] Cytotoxic Glycosylated Fatty Acid Amides from a Stelletta sp. Marine Sponge (2015)
  • Author(s): V. Peddie, K. Takada, S. Okuda, Y. Ise, Y. Morii, N. Yamawaki, T. Takatani, O. Arakawa,⊥ S. Okada, and S. Matsunaga
  • Journal Title: Journal of Natural Products Volume: 78 Issue: 11 Pages: 2808-2813
  • DOI: 10.1021/acs.jnatprod.5b00795
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural reappraisal of corticatic acids, biologically active linear polyacetylenes, from a marine sponge of the genus Petrosia (2014)
  • Author(s): K. Takada, S. Okada, S. Matsunaga
  • Journal Title: Fish. Sci. Volume: 80 Issue: 5 Pages: 1057-1064
  • DOI: 10.1007/s12562-014-0776-0
  • NAID: 40020207530
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Lower Homologues of Ahpatinin, Aspartic Protease Inhibitors, from a Marine Streptomyces sp. (2014)
  • Author(s): Y. Sun, K. Takada, Y. Nogi, S. Okada, S. Matsunaga
  • Journal Title: J. Nat. Prod. Volume: 77 Issue: 7 Pages: 1749-1752
  • DOI: 10.1021/np500337m
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Sequestered Fulvinol-related Polyacetylenes in Peltodoris atromaculata. (2014)
  • Author(s): Ciavatta M.L., Nuzzo, G., Takada K., Mathieu V., Kiss R., Villani G, Gavagnin M.
  • Journal Title: Journal of Natural Products Volume: 77 Issue: 7 Pages: 1678-1684
  • DOI: 10.1021/np500298h
  • Peer Reviewed
• [Journal Article] An environmental bacterial taxon with a large and distinct metabolic repertoire (2014)
  • Author(s): Wilson, M. C., Mori, T., Ruckert, C., Uria, A. R., Helf, M. J., Takada, K., Gernert, C., Steffens, U. A. E., Heycke, N., Schmitt, S., Rinke, C., Helfrich, E. J. N., Brachmann, A. O., Gurgui, C., Wakimoto. T., Kracht, M., Crusemann, M., Hentschel, U., Abe, I., Matsunaga, S., Kalinowski, J., Takeyama, H., Piel, J.
  • Journal Title: Nature Volume: 506 Issue: 7486 Pages: 58-62
  • DOI: 10.1038/nature12959
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Revised structure of cyclolithistide A, a cyclic depsipeptide from the marine sponge Discodermia japonica (2014)
  • Author(s): H. Tajima, T. Wakimoto, K. Takada, Y. Ise, I. Abe
  • Journal Title: J. Nat. Prod. Volume: 77 Issue: 1 Pages: 154-158
  • DOI: 10.1021/np400668k
  • Peer Reviewed
• [Journal Article] On the Assignment of the Absolute Configuration at the Isolated Methyl Branch in Miyakosyne A, Cytotoxic Linear Acetylene, from the Deep-Sea Marine Sponge Petrosia sp. (2013)
  • Author(s): Hitora, Y.; Takada, K.; Matsunaga, S.
  • Journal Title: Tetrahedron Volume: 69 Issue: 52 Pages: 11070-11073
  • DOI: 10.1016/j.tet.2013.11.013
  • Peer Reviewed
• [Journal Article] Cycloforskamide, a cytotoxic macrocyclic peptide from the sea slug Pleurobranchus forskalii (2013)
  • Author(s): K. C. Tan, T. Wakimoto, K. Takada, T. Ohtsuki, N. Uchiyama, Y. Goda, I. Abe
  • Journal Title: J. Nat. Prod. Volume: 76 Issue: 7 Pages: 1388-1391
  • DOI: 10.1021/np400404r
  • Peer Reviewed
• [Journal Article] Surugamides, Cyclic Octapeptides with Four D-Amino Acid Residues, from a Marine Streptomyces sp.: LCMS-Aided Inspection of Partial Hydrolysates for the Distinction of D- and L-Amino Acid Residues in the Sequence (2013)
  • Author(s): Takada, K.; Ninomiya A.; Naruse, M.; Sun, Y.; Miyazaki, M.; Nogi, Y.; Okada, S. Matsunaga, S.
  • Journal Title: J. Org. Chem. Volume: 78 Issue: 13 Pages: 6746-6750
  • DOI: 10.1021/jo400708u
  • Peer Reviewed
• [Journal Article] Stimulators of adiponenesis from the marine sponge Xestospongia testudinaria (2013)
  • Author(s): Akiyama, T.; Takada, K.; Oikawa, T.; Matsuura, N.; Ise, Y.; Okada, S.; Matsunaga, S.
  • Journal Title: Tetrahedron Volume: 69 Issue: 32 Pages: 6560-6564
  • DOI: 10.1016/j.tet.2013.06.007
  • Peer Reviewed
• [Journal Article] Probable Novel MEP Pathway Inhibitor and Its Binding Protein, IspG (2013)
  • Author(s): Nakagawa K, Takada K, Imamura N
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 77 Issue: 7 Pages: 1449-1454
  • DOI: 10.1271/bbb.130094
  • NAID: 10031190451
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] カイメン由来の多彩な二次代謝産物と共生微生物の関係 (2013)
  • Author(s): 高田 健太郎、松永茂樹
  • Journal Title: 化学と生物 Volume: 51 Pages: 333-339
  • NAID: 10031170702
• [Presentation] Phoslactomycin-type compounds from a marine Streptomyces sp. A232. (2016)
  • Author(s): Kentaro Takada, Yi Sun, Rogie R.Z. Carandang, Yuichi Nogi, Shigeki Matsunaga
  • Organizer: Gordon Conference
  • Place of Presentation: Ventura (U.S.A.)
  • Year and Date: 2016-03-09
  • Int'l Joint Research
• [Presentation] Sponge-metabolomics for the discovery of new cytotoxic constituents. (2015)
  • Author(s): Kentaro Takada
  • Organizer: Pacifichem
  • Place of Presentation: Honolulu (U.S.A.)
  • Year and Date: 2015-12-18
  • Int'l Joint Research
• [Presentation] カイメンメタボロミクスの有用物質探索への適用 (2015)
  • Author(s): 高田 健太郎
  • Organizer: マリンケミカルバイオロジー研究会
  • Place of Presentation: 東京海洋大学（東京）
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] 海綿動物の分類から見る共生微生物および天然物の関係 (2015)
  • Author(s): 高田 健太郎
  • Organizer: 日本分類学会連合
  • Place of Presentation: 国立科学博物館（東京）
  • Year and Date: 2015-01-08
  • Invited
• [Presentation] 3.Sponge Metabolomics and Challenges for Identification of the Producer of Marine Natural Products (2014)
  • Author(s): Kentaro Takada
  • Organizer: Korean Society of Pharmacognosy
  • Place of Presentation: Suwon (Korea)
  • Year and Date: 2014-10-30
  • Invited
• [Presentation] カイメンメタボロミクスから難培養性共生微生物への挑戦 (2014)
  • Author(s): 高田 健太郎
  • Organizer: 天然物パワーシンポジウム
  • Place of Presentation: 東京大学（東京）
  • Year and Date: 2014-07-19
  • Invited
• [Presentation] 多彩な海洋天然物と共生微生物の関係
  • Author(s): 高田 健太郎
  • Organizer: 天然物化学研究会
  • Place of Presentation: 東京農業大学（東京）
  • Invited