| Budget Amount *help |
¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2013: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
The aim of this study is to elucidate the mechanisms of growth stimulation of lactic acid bacteria by neurotransmitter, namely neurobiotics. The genome DNA of neurobiotics belonging to the genus Lactobacillus species, such as Lactobacillus hokkaidonensis, L. plantarum and L. buchneri contains several genes associated with the bacterial two-component systems. After feeding with a diet including certain neurobiotics for cows, rumen samples were collected and applied to the metagenomic analysis based on bacterial 16S rRNA genes. Metagenomic data from rumen samples clearly showed that the genus Lactobacillus was detected only in the rumen samples from cows with a necrobiotic diet, and that the copy numbers of 16S rRNA genes were significantly increased in the samples. This could contribute to the production of new functional foods and feeds with useful abilities such as maintenance of intestinal microflora.
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