Roles of the GARP complex during mammalian development

• Project/Area Number: 25712034
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Kumamoto University (2014-2016); Institute of Physical and Chemical Research (2013)
• Principal Investigator: Sugimoto Michihiko 熊本大学, 生命資源研究・支援センター, 助教 (10373317)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000); Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2014: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2013: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: マウス / 初期発生 / 多能性幹細胞 / 細胞分化 / 組織発生 / 細胞内小胞輸送 / 発生・分化 / 遺伝学 / 動物

Outline of Final Research Achievements

To elucidate roles of GARP complex during mammalian development, we obtained previously established mutant mouse lines for components of the GARP complex and generated knockout mouse lines for genes that we newly identified as components of the GARP complex. From phenotypes of mutant embryos, we found that these genes could be classified into three groups. These results suggest that the GARP complex plays various roles by exchanging components during mammalian development. Furthermore, we carried out liver specific Vps52 knockout analysis for a model for identification of the role of the GARP complex during tissue development. Liver specific Vps52 knockout embryos showed very similar phenotypes to liver specific knockout embryos for an autophagy related gene. These results provide new evidence that the GARP complex seems to regulate autophagy.

Research Products

• [Journal Article] Conserved role of Ovo in germline development in mouse and Drosophila. (2017)
  • Author(s): Hayashi, M., Shinozuka, Y., Shigenobu, S., Sato, M., Sugimoto, M., Ito, S., Abe, K., Kobayashi, S.
  • Journal Title: Scientific Report Volume: 6 Issue: 1 Pages: 40056-40056
  • DOI: 10.1038/srep40056
  • NAID: 120007135169
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cellular dynamics of mouse trophoblast stem cells: Identification of a persistent stem cell type. (2016)
  • Author(s): Motomura K, Oikawa M, Hirose M, Honda A, Togayachi S, Miyoshi H, Ohinata Y, Sugimoto M, Abe K, Inoue K, Ogura A.
  • Journal Title: Biology of Reproduction Volume: 94 Issue: 6 Pages: 122-122
  • DOI: 10.1095/biolreprod.115.137125
  • Peer Reviewed / Open Access
• [Journal Article] A Simple and Robust Method for Establishing Homogeneous Mouse Epiblast Stem Cell Lines by Wnt Inhibition (2015)
  • Author(s): Sugimoto M, Kondo M, Koga Y, Shiura H, Ikeda R, Hirose M, Ogura A, Murakami A, Yoshiki A, Chuva de Sousa Lopes SM, Abe K
  • Journal Title: Stem Cell Reports Volume: 4 Issue: 4 Pages: 744-757
  • DOI: 10.1016/j.stemcr.2015.02.014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Developmental genetics of the mouse <i>t</i>-complex (2014)
  • Author(s): Sugimoto M
  • Journal Title: Genes & Genetic Systems Volume: 89 Issue: 3 Pages: 109-120
  • DOI: 10.1266/ggs.89.109
  • NAID: 130004703729
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access
• [Presentation] A Simple and Robust Method for Establishing Homogeneous Mouse Epiblast Stem Cell Lines by Wnt Inhibition (2016)
  • Author(s): Michihiko Sugimoto, Masayo Kondo, Yumiko Koga, Rieko Ikeda, Susana M. Chuva de Sousa Lopes, Kuniya Abe
  • Organizer: 2016 AMMRA & AMPC meeting
  • Place of Presentation: Hakone, Japan
  • Year and Date: 2016-05-20
  • Int'l Joint Research
• [Presentation] WNT inhibition facilitates the establishment of stable and homogeneous EpiSCs (2015)
  • Author(s): Michihiko Sugimoto, Masayo Kondo, Yumiko Koga, Rieko Ikeda, Susana M. Chuva de Sousa Lopes, Kuniya Abe
  • Organizer: 29th International Mammalian Genome Conference
  • Place of Presentation: 横浜
  • Year and Date: 2015-11-08
  • Int'l Joint Research
• [Presentation] EpiSC樹立におけるWntシグナル抑制の効果 (2015)
  • Author(s): 杉本道彦、近藤昌代、古賀裕美子、池田理恵子、Chuva de Sousa Lopes S.M.、阿部訓也
  • Organizer: 日本遺伝学会第87回大会
  • Place of Presentation: 仙台
  • Year and Date: 2015-09-24
• [Presentation] tハプロタイプ胚性致死変異tw5の同定 -発生制御因子としてのVps52の機能解明を目指して- (2015)
  • Author(s): 杉本道彦
  • Organizer: 第29回モロシヌス研究会
  • Place of Presentation: 有馬
  • Year and Date: 2015-07-03
• [Presentation] Wnt signal inhibition facilitates the establishment of stable and homogeneous epiblast stem cell (EpiSC) lines (2015)
  • Author(s): Michihiko Sugimoto, Masayo Kondo, Yumiko Koga, Rieko Ikeda, Susana M. Chuva de Sousa Lopes, Kuniya Abe
  • Organizer: 48th Annual Meeting of the Japanese Society of Developmental Biologist
  • Place of Presentation: つくば
  • Year and Date: 2015-06-02
• [Presentation] Vps52および関連遺伝子のマウス胚発生における役割 (2014)
  • Author(s): 杉本道彦、田村勝、阿部訓也
  • Organizer: 日本遺伝学会第86回大会
  • Place of Presentation: 滋賀県 長浜バイオ大学
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] マウスt-complex致死変異より同定されたVps52の発生制御遺伝子としての役割 (2013)
  • Author(s): 杉本道彦、近藤昌代、廣瀬美智子、鈴木操、目加田和之、小倉淳郎、Artzt Karen、阿部訓也
  • Organizer: 第60回日本実験動物学会
  • Place of Presentation: 茨城県つくば市
• [Presentation] Molecular identification of tw5: Vps52 promotes pluripotential cell differentiation through cell interactions (2013)
  • Author(s): Michihiko Sugimoto, Masayo Kondo, Michiko Hirose, Misao Suzuki, Kazuyuki Mekada, Atsuo Ogura, Karen Artzt, Kuniya Abe
  • Organizer: 第46回日本発生生物学会
  • Place of Presentation: 島根県松江市
• [Presentation] 多能性細胞の分化制御因子としてのVps52遺伝子の役割 (2013)
  • Author(s): 杉本道彦
  • Organizer: 日本遺伝学会第85回大会
  • Place of Presentation: 神奈川県横浜市