Development of mitochondrial fucntional imaging using electron mediators as a probe
| Project/Area Number |
25713004
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyushu University |
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Principal Investigator |
Hyodo Fuminori 九州大学, 学内共同利用施設等, 准教授 (10380693)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Keywords | レドックス / イメージング / ミトコンドリア / DNP / 動的核偏極 / フリーラジカル / MRI / ラジカル / ＭＲＩ / 活性酸素 / ＤＮＰ |
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| Outline of Final Research Achievements |
In vivo eedox reactions that generate free radical intermediates are essential to metabolic processes on homeostasis. We report here the use of in vivo dynamic nuclear polarization-magnetic resonance imaging (DNP-MRI) to conduct redox molecular imaging for mitochondrial functional imaging. Using in vivo DNP-MRI, we obtained simultaneous images of free radical intermediates generated from the CoQ10, FMN, and FAD involved in the mitochondrial electron transport chain, as well as the radicals derived from vitamins E and K1. Each of these free radicals was imaged in real time in a phantom comprising a mixture of free radicals localized in either lipophilic or aqueous environments. In addition, CoQH as a in vivo probe could be visualized redox alteration after mitochondrial dysfunction by KCN treatment. This study is the first to report the in vivo DNP-MRI imaging of free radical intermediates that are derived from endogenous species involved in metabolic processes.
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Report
(4 results)
Research Products
(17 results)
