| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Outline of Final Research Achievements |
Whole genome sequencing of clinical isolates of Acinetobacter baumannii collected in Japan was performed. Comparative genome-wide association studies using A. baumannii isolates collected and isolates in public databases was performed to identify genetic traits of the pandemic lineages. Most of the pandemic lineages conserved an intact type VI secretion system (T6SS) gene cluster, whereas drug-susceptible non-epidemic isolates frequently lacked the gene clusters. Whole-genome single nucleotide polymorphism-based phylogenetic analysis revealed that pandemic international clone II (IC2) isolates are further divided into several distinct genetic clades. Comparative genomic analysis between IC2 isolates identified that each IC2 clade harbors own specific antibacterial T6SS substrates. Given that T6SS-mediated bacterial competition is known to affect conjugative transfer of drug resistance genes-carrying plasmids, T6SS could be an important key factor for development of high-risk clones.
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