Construction of mass spectrometry based rapid cancer diagnosis system

• Project/Area Number: 25713023
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: University of Yamanashi
• Principal Investigator: YOSHIMURA Kentaro 山梨大学, 総合研究部, 助教 (70516921)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000); Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000); Fiscal Year 2014: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 機械学習 / バルクデータ / がん診断 / 大気圧イオン化法 / リアルタイム質量分析 / メタボロミクス / 学習機械 / バルクデータ解析 / オンサイト質量分析 / 迅速診断 / オンサイト分析

Outline of Final Research Achievements

We constructed the rapid diagnosis system for human renal cell carcinoma and hepatocellular carcinoma using probe electrospray ionization-mass spectrometry (PESI-MS) and learning machine, and achieved over 90% coincidence of diagnosis with those judged by the conventional histopathology. To validate the applicability of this system, we applied PESI-MS to diagnosis of various cancers. In this study duration, we could collect the 22,000 mass spectra from glioma, oral squamous cell carcinoma, gastric cancer, colonic cancer, bladder cancer, and pancreatic cancer.

Research Products

• [Journal Article] Modulation of primary cilia length by melanin-concentrating hormone receptor 1. Cell Signaling (2016)
  • Author(s): Hamamoto A, Yamato S, Katoh Y, Nakayama K, Yoshimura K, Takeda S, Kobayashi Y, *Saito Y
  • Journal Title: Cellular Signalling Volume: 28 Issue: 6 Pages: 272-584
  • DOI: 10.1016/j.cellsig.2016.02.018
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 相反する視座からの新しいがん診断支援装置の試み臨床病理 (2015)
  • Author(s): 竹田 扇、吉村 健太郎、田邉 國士
  • Journal Title: 臨床病理 Volume: 63 Pages: 1083-1089
• [Journal Article] Involvement of FKBP6 in hepatitis C virus replication. (2015)
  • Author(s): Kasai H, Kawakami Y, Yokoe H, Yoshimura K, Matsuda M, Yasumoto J, Maekawa S, Yamashita A, Tanaka T, Ikeda M, Kato N, Okamoto T, Matsuura Y, Sakamoto N, Enomoto N, Takeda S, Fujii H, Tsubuki M, Kusunoki M, Moriishi K.
  • Journal Title: Scientific reports Volume: 5 Issue: 1 Pages: 16699-16699
  • DOI: 10.1038/srep16699
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Untreated and dried sample analysis by solid probe assisted nanoelectrospray ionization mass spectrometry (2015)
  • Author(s): Mridul Kanti Mandal, Subhrakanti Saha, Zhan Yu, Kentaro Yoshimura, Sen Takeda, Kenzo Hiraoka
  • Journal Title: Analytical Methods Volume: 7 Pages: 2630-2635
  • Peer Reviewed
• [Journal Article] Development of Non-proximate Probe Electrospray Ionization for Real-Time Analysis of Living Animal (2014)
  • Author(s): Kentaro Yoshimura, Lee Chuin Chen, Hisashi Johno, Mayutaka Nakajima, Kenzo Hiraoka, Sen Takeda
  • Journal Title: Mass Spectrometry Volume: 3 Pages: 1-7
  • NAID: 130004972836
  • Peer Reviewed / Open Access
• [Journal Article] 質量分析法と統計的学習機械を組み合わせた新規がん診断支援装置の開発 (2013)
  • Author(s): 竹田 扇、吉村 健太郎、出水 秀明、平岡 賢三、谷畑 博司、田邉 國士、中島 宏樹、堀 裕和
  • Journal Title: 島津評論 Volume: 69 Pages: 203-210
• [Journal Article] Real-time Diagnosis of Chemically Induced Hepatocellular Carcinoma Us ing a Novel Mass Spectrometry-based Technique (2013)
  • Author(s): K. Yoshimura, M. K. Mandal, M . Hara, H. Fujii, L. C. Chen, K. Tanabe , K. Hiraoka and S. Takeda
  • Journal Title: Analytical Biochemistry Volume: VOL.441 Issue: 1 Pages: 32-37
  • DOI: 10.1016/j.ab.2013.06.017
  • Peer Reviewed
• [Journal Article] Direct Electrospray Ionization Mass Spectrometric Profiling of Real-World Samples via a Solid Sampling Probe (2013)
  • Author(s): Z. Yu, L. C. Chen, M. K. Mand al, K. Yoshimura, S. Takeda and K. Hiraoka
  • Journal Title: Journal of The American Society for Mass Spectr ometry Volume: VOL.24 Issue: 10 Pages: 1612-1615
  • DOI: 10.1007/s13361-013-0697-7
  • Peer Reviewed
• [Journal Article] Biomolecular Analysis and Biological Tissue Diagnostics by Electrospray Ionization with a Metal Wire Inserted Gel-Loading Tip (2013)
  • Author(s): M. K. Mandal, K. Yoshimua, S. Saha, Z. Yu, S. Takeda and K. Hiraoka.
  • Journal Title: Analytical Chemistry Volume: VOL.86 Issue: 2 Pages: 987-992
  • DOI: 10.1021/ac403261s
  • Peer Reviewed
• [Presentation] ミエリン形成におけるヘッジホッグシグナルの機能とシグナル活性調節機構の解明 (2016)
  • Author(s): 吉村 健太郎
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグバレットふくしま（福島県・郡山市）
  • Year and Date: 2016-03-28
• [Presentation] Hedgehog signaling specifically regulates the morphogenesis of Schwann cell in promyelinating stage (2015)
  • Author(s): 吉村 健太郎
  • Organizer: 第38回 日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-30
• [Presentation] Development of real-time metabolomic analysis system and database construction/statistical analysis software (2015)
  • Author(s): 吉村 健太郎
  • Organizer: 第63回質量分析総合討論会
  • Place of Presentation: つくば国際会議場エポカルつくば（茨城県・つくば市）
  • Year and Date: 2015-06-19
• [Presentation] Developing a cancer diagnostics system: Towards on-site multi purpose gadgetry (2014)
  • Author(s): K.Yoshimura, L.C.Chen, K.Tanabe, M. Nakajima, S. Ninomiya, H. Hori, H. Izumi, K. Hiraoka, S. Takeda
  • Organizer: 20th International Mass Spectrometry Conference
  • Place of Presentation: Centre International de Confrences Genve (Swiss)
  • Year and Date: 2014-08-25
• [Presentation] Development and Application of Diagnosis System Using Probe Electrospray Ionization and Dual Penalized Logistic Regression Machine (2014)
  • Author(s): K.Yoshimura, K.Tanabe, L.C.Chen, S.Ninomiya, H.Hori, H.Izumi, K.Hiraoka, S.Takeda
  • Organizer: 第62回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク (大阪府・吹田市)
  • Year and Date: 2014-05-14
• [Presentation] Human cancer diagnosis by the electrospray ionization and Bayesian statistics
  • Author(s): Kentaro Yoshimura, Kunio Tanabe, Lee Chuin Chen, Hiroki Nakajima, Kenzo Hiraoka, Sen Takeda
  • Organizer: 4th Asia Oceania Mass Spectrometry Conference
  • Place of Presentation: Taipei International Convention Center, Taipei, Taiwan
• [Presentation] ベイズ統計を用いた質量分析型がん診断装置の開発
  • Author(s): 吉村健太郎・田邉國士・Lee Chuin Chen・二宮啓・堀裕和・中島宏樹・平岡賢三・竹田扇
  • Organizer: 第61回質量分析総合討論会
  • Place of Presentation: つくば国際会議場エポカルつくば (茨城県、つくば市)
• [Presentation] 大気圧イオン化法および学習機械を用いた 質量分析型迅速診断システムの開発
  • Author(s): 吉村健太郎
  • Organizer: 大阪大学質量分析シンポジウム、大阪大学未来研究イニシアティブ・グループ支援事業講演会
  • Place of Presentation: 大阪大学理学研究科、H棟701号室 (大阪府、豊中市)
  • Invited
• [Remarks] 国立大学法人山梨大学研究者総覧
  • URL: http://erdb.yamanashi.ac.jp/rdb/A_DispDetail.Scholar
• [Remarks] 山梨大学 大学院医学工学総合研究部 解剖学講座細胞生物学教室
  • URL: http://www.cellbiology.jp
• [Remarks] 質量分析、メタボロミクス、データ解析
  • URL: http://hh-bd.link
• [Remarks] 国立大学法人山梨大学研究者総覧
  • URL: http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar/3_51/39A95025371B8030.html
• [Remarks] 山梨大学大学院医学工学総合研究部解剖学講座細胞生物学教室ホームページ
  • URL: http://www.cellbiology.jp/
• [Patent(Industrial Property Rights)] マススペクトル解析システム，方法およびプログラム (2015)
  • Inventor(s): 吉村健太郎、城野悠志、船山慧、川井将敬、森優喜、竹田扇
  • Industrial Property Rights Holder: 国立大学法人山梨大学
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2015-078992
  • Filing Date: 2015-04-08