Transcriptome of bacteria during host infection against autophagy

Research Project

Project/Area Number	25713060
Research Category	Grant-in-Aid for Young Scientists (A)
Allocation Type	Partial Multi-year Fund
Research Field	Morphological basic dentistry
Research Institution	Kyoto University (2014-2015) Tokyo Medical and Dental University (2013)
Principal Investigator	Maruyama Fumito 京都大学, 医学(系)研究科(研究院), 准教授 (30423122)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000) Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000) Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2013: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Keywords	トランスクリプトーム / ゲノム / レンサ球菌 / オートファジー / メチローム / 細菌 / 進化 / A群レンサ球菌
Outline of Final Research Achievements	There are three major findings as follows: i) Streptococcus pyogenes are diversified into two subspecies using CRISPR by restriction of number and kind of phage invasion into genome, ii) One subspecies may appear by step-wise deletion of CRISPR and final complete deletion of CRISPR/cas system by an altruistic phage, iii) virulent type of this bacteria carries smaller genome size compared to the avirulent type, which is not related to phylogeny but related to specific SNPs that could determine the virulence.