| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Many microbes are living by forming symbiotic systems that have complex structures and functions by communicating with other microbes. In order to understand the design principle of these symbiotic systems, we constructed a synthetic mutualistic system that is easy to be controlled and analyzed with the synthetic biological approach. Firstly, we designed and constructed synthetic genetic circuits that make E.coli cells to form a mutualistic system in which two types of cells help the other’s survival by inducing expression of antibiotic resistance genes through cell-cell communication molecules. Furthermore, we confirmed that the synthetic mutualistic system can exist stably with culture experiments and numerical simulations.
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