| Project/Area Number |
25740019
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
YOKOTA Yuichiro 国立研究開発法人日本原子力研究開発機構, 原子力科学研究部門 量子ビーム応用研究センター, 研究副主幹 (30391288)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | バイスタンダー効果 / ガンマ線 / 炭素イオンビーム / 一酸化窒素 / 炭素イオン / 遺伝子発現 / 重イオンビーム / 線量依存性 / 線質依存性 / 重イオン / carboxy-PTIO / ザルツマン法 |
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| Outline of Final Research Achievements |
To elucidate the mechanism of bystander effect, cells were irradiated with gamma-rays or carbon-ion beam. After irradiation, irradiated cells and non-irradiated cells were co-cultured in the same vessel. As a result, clonogenicity of non-irradiated cells decreased when the co-culture time with and the dose absorbed in irradiated cells increased. There was no significant difference in their clonogenicity between gamma-ray and carbon-ion irradiation. From these results, it has been clearly showed that bystander effect is dependent on co-culture time and absorbed dose, and independent of radiation quality. When a nitric-oxide scavenger was added during co-culture, reduction in clonogenicity disappeared. There were also negative relationships between the clonogenicity and the concentrations of nitrite, derived from nitric oxide, in co-culture medium. The yield of nitric oxide was, therefore, assumed to be one of the determinants of bystander effect.
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