Analysisi of genetic instability induction by the introduction of non-DSB damages

• Project/Area Number: 25740021
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Osaka Prefecture University
• Principal Investigator: Urushibara Ayumi 大阪府立大学, 理学(系)研究科(研究院), 客員研究員 (80391275)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 非DSB型DNA損傷 / 紫外線 / 遺伝的不安定性 / 染色体異常 / 核型異常 / DNA損傷 / 酸化損傷 / 非DSBs型クラスターDNA損傷 / 核型 / 細胞周期 / 生物影響

Outline of Final Research Achievements

Ionizing radiation induces genetic instability in the progeny of the irradiated cells, that is estimated to be involved in radiation carcinogenesis. To clarify the mechanism of genetic instability is a very important because even lead to the elucidation of carcinogenic processes not only the biological effects of radiation. In this study, we hypothesized that some of the non-DSB type of DNA damage remaining after DSB repair may be related to the induction of genetic instability. As one of the causes that induce genetic instability, it was focused on the type of non-DSB damage. Then, we analyzed using delayed chromosomal aberrations and karyotype in order to clarify the differences in the genetic instability induced by the type of difference in damage.

Research Products

• [Journal Article] Chemical repair activity of free radical scavenger, edaravone: Reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA. (2015)
  • Author(s): Hata K, Urushibara A, Yamashita S, Lin M, Muroya Y, Shikazono N, Yokoya A, Fu H, Katsumura Y
  • Journal Title: Journal of Radiation Research Volume: 56 Issue: 1 Pages: 59-66
  • DOI: 10.1093/jrr/rru079
  • NAID: 120006502225
  • Peer Reviewed / Open Access
• [Journal Article] Induction of genetic instability by transfer of a UV-A-irradiated chromosome (2014)
  • Author(s): Urushibara, A., Kodama, S., and Yokoya, A.
  • Journal Title: Mutat. Res Volume: 766 Pages: 29-34
  • DOI: 10.1016/j.mrgentox.2014.02.005
  • Peer Reviewed
• [Journal Article] Significance of DNA Polymerase I in in vivo processing of clustered DNA damage (2013)
  • Author(s): Shikazono, N., Akamatsu, K., Takahashi, M., Noguchi, M., Urushibara, A., O’Neill, P. and Yokoya, A.
  • Journal Title: Mutatation Research Volume: 749 Issue: 1-2 Pages: 9-15
  • DOI: 10.1016/j.mrfmmm.2013.07.010
  • Peer Reviewed
• [Journal Article] Chemical repair of base lesions, AP-sites, and strand breaks on plasmid DNA in dilute aqueous solution by ascorbic acid (2013)
  • Author(s): Hata K, Urushibara A, Yamashita S, Shikazono N, Yokoya A, Katsumura Y
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 434 Issue: 2 Pages: 341-345
  • DOI: 10.1016/j.bbrc.2013.03.075
  • Peer Reviewed
• [Remarks] 日本原子力研究開発機構：プレス発表 DNA損傷が正常な染色体にも影響を与えることを発見(お知らせ)
  • URL: https://www.jaea.go.jp/02/press2014/p14041801/
• [Remarks] 漆原あゆみ客員研究員、児玉靖司教授の共同研究「DNA損傷が正常な染色体にも影響を与えることを発見」
  • URL: http://www.osakafu-u.ac.jp/affiliate/2014/nws20140422.html