Quantitative evaluation of radiation-induced response on tissue stem cells
Project/Area Number |
25740022
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Risk sciences of radiation and chemicals
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Research Institution | Central Research Institute of Electric Power Industry |
Principal Investigator |
Kensuke Otsuka 一般財団法人電力中央研究所, 原子力技術研究所, 主任研究員 (50371703)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 組織幹細胞 / DNA損傷 / 腸管 / 低線量放射線 / 細胞動態 / EdU / フローサイトメトリー / 放射線 / 放射線発がん / Lgr5 / 蛍光免疫染色 / 組織ホールマウント |
Outline of Final Research Achievements |
The accumulation of DNA damage in the intestinal stem cells may be crucial for the development of cancer initiating cells. Therefore, it is important to understand the kinetics of DNA repair and tissue response, which is involved in the elimination of damaged cells and tissue injury repair, in response to irradiation. In this study, DNA damage response and cellular dynamics on intestinal stem cells was determined by quantitative protocols through whole-mount single crypt immunofluorescent and flow cytometry. DNA damage repair and the elimination of damaged cells were quantified by measuring the 53BP1 and gamma-H2AX foci formation. Tissue proliferative response was evaluated by population of EGFP-positive cells and determining the EdU incorporating cells. The result indicated that radiation-induce DNA damage was repaired efficiently in Lgr5+ stem cells.
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Report
(4 results)
Research Products
(12 results)
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[Journal Article] Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after γ-irradiation through Wnt/β-catenin pathway.2015
Author(s)
Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura K, Nakagama H, Mishima Y, and Kominami R.
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Journal Title
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Peer Reviewed
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