Analysis of human thyroid damage induced by radioactive iodine using Super-SCID mice

• Project/Area Number: 25740023
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: ADACHI Shigeki 独立行政法人医薬基盤研究所, 難病・疾患資源研究部, 研究員 (60379261)
• Research Collaborator: TORI Masayuki ; NOMURA Taisei
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 放射性ヨウ素 / ヒト甲状腺 / SCIDマウス / ヒト甲状腺移植 / 遺伝子発現 / I-131 / マイクロアレイ / マイクロアレイ解析

Outline of Final Research Achievements

80％ of fatal risk to human by radiation is cancer. Thyroid cancer developed in high frequency by Chernobyl Nuclear Power Plant accident. Human thyroid disorder is feared after the Fukushima Daiichi nuclear accident. By the injection of I-131 to Super-SCID mouse with human thyroid, it is aimed to clarify the direct influence of I-131 to human thyroid by internal exposure.
Human thyroid tissues were transplanted to Super-SCID mice, and 0.5 and 0.0625MBq of I-131 was injected i.p. to Super-SCID mice with human thyroid tissues. 1 and 2 weeks after I-131 injection, RNA was extracted from transplanted thyroid tissue and analyzed by microarray. 4-fold changes tended to increase with time. There were common two genes which showed more than 4-fold change in the thyroid tissues injected high dose(0.5、0.25MBq) I-131. It was COL8A1 and HSD17B6.

Research Products

• [Journal Article] Absence of lactobacilli containing glycolipids with the α-galactose epitope and the enhanced fucosylation of a receptor glycolipid GA1 in the digestive tracts of immune-deficient scid mice. (2015)
  • Author(s): Iwamori M, Tanaka K, Adachi S, Aoki D, Nomura T.
  • Journal Title: J Biochem Volume: pii Issue: 1 Pages: 73-82
  • DOI: 10.1093/jb/mvv021
  • Peer Reviewed
• [Journal Article] Histological Evaluation of Human Benign Prostatic Hyperplasia Treated by Dutasteride: A Study by Xenograft Model with Improved Severe Combined Immunodeficient (Super-SCID) Mice. (2015)
  • Author(s): Akira Tsujimura, Shinichiro Fukuhara, Tetsuji Soda, Kentaro Takezawa, Hiroshi Kiuchi, Tetsuya Takao, Yasushi Miyagawa, Norio Nonomura, Shigeki Adachi, Yoriko Tokita, Taisei Nomura.
  • Journal Title: Urology Volume: 85 Issue: 1 Pages: 274.e1-274.e8
  • DOI: 10.1016/j.urology.2014.09.024
  • Peer Reviewed
• [Journal Article] Altered Actions of Memantine and NMDA-Induced Currents in a New Grid2-Deleted Mouse Line. (2014)
  • Author(s): Ayako Kumagai, Akira Fujita, Tomoki Yokoyama, Yuki Nonobe, Yasuhiro Hasaba, Tsutomu Sasaki, Yumi Itoh, Minako Koura, Osamu Suzuki, Shigeki Adachi, Haruko Ryo, Arihiro Kohara, Lokesh P. Tripathi, Masato Sanosaka, Toshiki Fukushima, Taisei Nomura, Hiroshi Takemori, et al.
  • Journal Title: Genes Volume: 5 Issue: 4 Pages: 1095-1114
  • DOI: 10.3390/genes5041095
  • Peer Reviewed / Open Access
• [Presentation] ヒトがん組織等移植SCIDマウスを用いた重粒子線の有効性・安全性の研究 (2015)
  • Author(s): 野村大成、足立成基、梁 治子、坂巻 靖、鳥 正幸、吉留克英、野々村祝夫、古澤佳也、鵜澤玲子
  • Organizer: 平成26年度 HIMAC共同利用研究 成果発表会
  • Place of Presentation: ホテルポートプラザちば
  • Year and Date: 2015-04-20
• [Presentation] 乳酸菌の腸内共生と免疫の役割 (2014)
  • Author(s): 岩森 正男、足立 成基、野村 大成、田中 京子、青木 大輔
  • Organizer: 第56回 日本脂質生化学会
  • Place of Presentation: 近畿大学東大阪キャンパス
  • Year and Date: 2014-06-07