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Development of hypoallergenic methods of a major fish allergen, parvalbumin

Research Project

Project/Area Number 25750023
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Kobayashi Yukihiro  東京海洋大学, その他部局等, 助教 (30511753)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsパルブアルブミン / 加熱 / IgE / 変性 / 立体構造 / Immunoglobulin E (IgE) / カルシウム / 魚類 / アレルゲン / 食物アレルギー
Outline of Final Research Achievements

It has been believed that allergenicity of a major fish allergen, parvalbumin (PV) is highly heat-stable. However, we revealed that IgE-binding sites, which are important for eliciting of allergies, exist on a conformation of PV, and it was predicted a possibility of reduction of allergenicity by denaturation of PV using heat treatment. Although heating load, such as usual cooking, did not reduced allergenicity of PV, Heavy heating load, e.g. at 100℃ for 320 min, at 120℃ for 60 min, and at 140℃ for 10 min, completely reduced allergenicity of PV. In addition, we revealed that heat treatment generated collapse of the conformational structure of PV. Furthermore, we elucidated that high heating loads can reduce allergenicities of PV from various fish species.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013

All Journal Article (10 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 8 results,  Open Access: 3 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Fish collagen is an important panallergen in the Japanese population2016

    • Author(s)
      Kobayashi Y, Akiyama H, Huge J, Kubota H, Chikazawa S, Satoh T, Miyake T, Uhara H, Okuyama R, Nakagawara R, Aihara M, Hamada-Sato N
    • Journal Title

      Allergy

      Volume: 未定 Issue: 5 Pages: 720-723

    • DOI

      10.1111/all.12836

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Study of the cross-reactivity of fish allergens based on a questionnaire and blood testing2016

    • Author(s)
      Kobayashi Y, Huge J, Imamura S, Hamada-Sato N
    • Journal Title

      Allergology International

      Volume: 65 Issue: 3 Pages: 272-279

    • DOI

      10.1016/j.alit.2016.01.002

    • NAID

      130005165864

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Quantification of major allergen parvalbumin in 22 species of fish by SDS-PAGE2016

    • Author(s)
      Kobayashi Y, Yang T, Yu CT, Ume C, Kubota H, Shimakur K, Shiomi K, Hamada-Sato N
    • Journal Title

      Food Chemistry

      Volume: 194 Pages: 345-353

    • DOI

      10.1016/j.foodchem.2015.08.037

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Reduction in IgE reactivity of Pacific mackerel parvalbumin by heat treatment2016

    • Author(s)
      Kubota H, Kobayashi A, Kobayashi Y, Shiomi K, Hamada-Sato N
    • Journal Title

      Food Chemistry

      Volume: 206 Pages: 78-84

    • DOI

      10.1016/j.foodchem.2016.03.043

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] IgE-binding epitopes of various fish parvalbumins exist in a stereoscopic conformation maintained by Ca<sup>2+</sup> binding2016

    • Author(s)
      Kobayashi A, Ichimura A, Kobayashi Y, Shiomi K
    • Journal Title

      Allergology International

      Volume: 65 Issue: 3 Pages: 345-348

    • DOI

      10.1016/j.alit.2016.02.004

    • NAID

      130005165870

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Fish allergy in patients with parvalbumin -specific immunoglobulin E depends on parvalbumin content rather than molecular differences in the protein among fish.2016

    • Author(s)
      Kobayashi A, Kobayashi Y, Shiomi K.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry.

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] IgE-binding epitopes of various fish parvalbumins exist in a stereoscopic conformation maintained by Ca2+ binding.2016

    • Author(s)
      Kobayashi A, Ichimura A, Kobayashi Y, Shiomi K.
    • Journal Title

      Allergology International

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 魚類主要アレルゲンパルブアルブミンのアレルゲン性の低減化2015

    • Author(s)
      小林征洋
    • Journal Title

      アレルギーの臨床

      Volume: 35 Pages: 1158-1163

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] イタリアンレストランの調理師に生じた魚類アレルギーの1例~魚類アレルギーにおける経皮感作の危険性~.2015

    • Author(s)
      原田 晋, 小林征洋, 塩見一雄, 松本玲子.
    • Journal Title

      Journal of Environmental Dermatology and Cutaneous Allergology

      Volume: 9 Pages: 179-186

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] パルブアルブミンによる魚類アレルギーの2例2014

    • Author(s)
      篠原理恵, 藤本和久, 小林征洋
    • Journal Title

      皮膚科の臨床

      Volume: 56 Pages: 1270-1274

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] ウナギ目の魚類によるアナフィラキシーの1例2015

    • Author(s)
      白川典子, 藤本和久, 佐伯秀久, 小林征洋
    • Organizer
      第45回日本皮膚アレルギー・接触皮膚炎学会総会
    • Place of Presentation
      島根
    • Year and Date
      2015-11-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] アンケート調査およびアレルギー反応性に基づく魚類アレルゲンの抗原交差性2015

    • Author(s)
      小林征洋, 呼格吉楽図, 今村慎太郎, 濱田(佐藤)奈保子
    • Organizer
      日本食品衛生学会第110回学術講演会
    • Place of Presentation
      京都
    • Year and Date
      2015-10-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 魚によるFDEIAの1例~当科の魚アレルギーの検討を含めて~2015

    • Author(s)
      南知里, 李和純, 仲田かおり, 錦織千佳子, 小林征洋
    • Organizer
      第114回日本皮膚科学会総会学術大会
    • Place of Presentation
      横浜
    • Year and Date
      2015-05-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 加熱処理によるマサバパルブアルブミンのアレルゲン性低減化.2014

    • Author(s)
      久保田宙幸, 小林征洋, 濱田(佐藤)奈保子.
    • Organizer
      日本食品衛生学会第108回学術講演会
    • Place of Presentation
      金沢
    • Year and Date
      2014-12-03 – 2014-12-06
    • Related Report
      2014 Research-status Report
  • [Presentation] 魚類摂取によるアレルギー~魚類アレルギーおよびアニサキスアレルギー~2014

    • Author(s)
      小林征洋
    • Organizer
      第43回兵庫県臨床アレルギー研究会
    • Place of Presentation
      神戸
    • Year and Date
      2014-11-01
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 調味料および加熱処理がマサバ主要アレルゲン(パルブアルブミン)に与える影響.2013

    • Author(s)
      馮奕,小林征洋,濱田奈保子.
    • Organizer
      日本食品科学工学会第60回記念大会
    • Place of Presentation
      日野
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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