Development of hypoallergenic methods of a major fish allergen, parvalbumin

• Project/Area Number: 25750023
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Kobayashi Yukihiro 東京海洋大学, その他部局等, 助教 (30511753)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: パルブアルブミン / 加熱 / IgE / 変性 / 立体構造 / Immunoglobulin E (IgE) / カルシウム / 魚類 / アレルゲン / 食物アレルギー

Outline of Final Research Achievements

It has been believed that allergenicity of a major fish allergen, parvalbumin (PV) is highly heat-stable. However, we revealed that IgE-binding sites, which are important for eliciting of allergies, exist on a conformation of PV, and it was predicted a possibility of reduction of allergenicity by denaturation of PV using heat treatment. Although heating load, such as usual cooking, did not reduced allergenicity of PV, Heavy heating load, e.g. at 100℃ for 320 min, at 120℃ for 60 min, and at 140℃ for 10 min, completely reduced allergenicity of PV. In addition, we revealed that heat treatment generated collapse of the conformational structure of PV. Furthermore, we elucidated that high heating loads can reduce allergenicities of PV from various fish species.

Research Products

• [Journal Article] Fish collagen is an important panallergen in the Japanese population (2016)
  • Author(s): Kobayashi Y, Akiyama H, Huge J, Kubota H, Chikazawa S, Satoh T, Miyake T, Uhara H, Okuyama R, Nakagawara R, Aihara M, Hamada-Sato N
  • Journal Title: Allergy Volume: 未定 Issue: 5 Pages: 720-723
  • DOI: 10.1111/all.12836
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Study of the cross-reactivity of fish allergens based on a questionnaire and blood testing (2016)
  • Author(s): Kobayashi Y, Huge J, Imamura S, Hamada-Sato N
  • Journal Title: Allergology International Volume: 65 Issue: 3 Pages: 272-279
  • DOI: 10.1016/j.alit.2016.01.002
  • NAID: 130005165864
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Quantification of major allergen parvalbumin in 22 species of fish by SDS-PAGE (2016)
  • Author(s): Kobayashi Y, Yang T, Yu CT, Ume C, Kubota H, Shimakur K, Shiomi K, Hamada-Sato N
  • Journal Title: Food Chemistry Volume: 194 Pages: 345-353
  • DOI: 10.1016/j.foodchem.2015.08.037
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Reduction in IgE reactivity of Pacific mackerel parvalbumin by heat treatment (2016)
  • Author(s): Kubota H, Kobayashi A, Kobayashi Y, Shiomi K, Hamada-Sato N
  • Journal Title: Food Chemistry Volume: 206 Pages: 78-84
  • DOI: 10.1016/j.foodchem.2016.03.043
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] IgE-binding epitopes of various fish parvalbumins exist in a stereoscopic conformation maintained by Ca²⁺ binding (2016)
  • Author(s): Kobayashi A, Ichimura A, Kobayashi Y, Shiomi K
  • Journal Title: Allergology International Volume: 65 Issue: 3 Pages: 345-348
  • DOI: 10.1016/j.alit.2016.02.004
  • NAID: 130005165870
  • ISSN: 1323-8930, 1440-1592
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fish allergy in patients with parvalbumin -specific immunoglobulin E depends on parvalbumin content rather than molecular differences in the protein among fish. (2016)
  • Author(s): Kobayashi A, Kobayashi Y, Shiomi K.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry. Volume: 未定
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] IgE-binding epitopes of various fish parvalbumins exist in a stereoscopic conformation maintained by Ca2+ binding. (2016)
  • Author(s): Kobayashi A, Ichimura A, Kobayashi Y, Shiomi K.
  • Journal Title: Allergology International Volume: 未定
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 魚類主要アレルゲンパルブアルブミンのアレルゲン性の低減化 (2015)
  • Author(s): 小林征洋
  • Journal Title: アレルギーの臨床 Volume: 35 Pages: 1158-1163
  • Acknowledgement Compliant
• [Journal Article] イタリアンレストランの調理師に生じた魚類アレルギーの１例～魚類アレルギーにおける経皮感作の危険性～. (2015)
  • Author(s): 原田 晋, 小林征洋, 塩見一雄, 松本玲子.
  • Journal Title: Journal of Environmental Dermatology and Cutaneous Allergology Volume: 9 Pages: 179-186
  • Peer Reviewed
• [Journal Article] パルブアルブミンによる魚類アレルギーの2例 (2014)
  • Author(s): 篠原理恵, 藤本和久, 小林征洋
  • Journal Title: 皮膚科の臨床 Volume: 56 Pages: 1270-1274
  • Peer Reviewed
• [Presentation] ウナギ目の魚類によるアナフィラキシーの1例 (2015)
  • Author(s): 白川典子, 藤本和久, 佐伯秀久, 小林征洋
  • Organizer: 第45回日本皮膚アレルギー・接触皮膚炎学会総会
  • Place of Presentation: 島根
  • Year and Date: 2015-11-20
• [Presentation] アンケート調査およびアレルギー反応性に基づく魚類アレルゲンの抗原交差性 (2015)
  • Author(s): 小林征洋, 呼格吉楽図, 今村慎太郎, 濱田（佐藤）奈保子
  • Organizer: 日本食品衛生学会第110回学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2015-10-29
• [Presentation] 魚によるFDEIAの1例～当科の魚アレルギーの検討を含めて～ (2015)
  • Author(s): 南知里, 李和純, 仲田かおり, 錦織千佳子, 小林征洋
  • Organizer: 第114回日本皮膚科学会総会学術大会
  • Place of Presentation: 横浜
  • Year and Date: 2015-05-29
• [Presentation] 加熱処理によるマサバパルブアルブミンのアレルゲン性低減化. (2014)
  • Author(s): 久保田宙幸, 小林征洋, 濱田(佐藤)奈保子.
  • Organizer: 日本食品衛生学会第108回学術講演会
  • Place of Presentation: 金沢
  • Year and Date: 2014-12-03 – 2014-12-06
• [Presentation] 魚類摂取によるアレルギー～魚類アレルギーおよびアニサキスアレルギー～ (2014)
  • Author(s): 小林征洋
  • Organizer: 第43回兵庫県臨床アレルギー研究会
  • Place of Presentation: 神戸
  • Year and Date: 2014-11-01
  • Invited
• [Presentation] 調味料および加熱処理がマサバ主要アレルゲン（パルブアルブミン）に与える影響. (2013)
  • Author(s): 馮奕，小林征洋，濱田奈保子.
  • Organizer: 日本食品科学工学会第60回記念大会
  • Place of Presentation: 日野