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Enterobacteriaceae and foodborne bacterial pathogen contamination of ready-to-eat foods retailed in Japan

Research Project

Project/Area Number 25750069
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Eating habits
Research InstitutionOsaka Prefectural Institute of Public Health

Principal Investigator

Harada Tetsuya  大阪府立公衆衛生研究所, その他部局等, 研究員 (70516723)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords非加熱摂取食品 / ready-to-eat foods / bacterial contamination / 腸内細菌科菌群
Outline of Final Research Achievements

To investigate the bacterial contamination of ready-to-eat (RTE) foods retailed in Japan, the prevalence of Enterobacteriaceae and foodborne bacterial pathogens in 96 Japanese light pickles, 88 western-style sweets, and 98 Japanese seafood products was detected in this study. Enterobacteriaceae was isolated from 92 (95.8%) Japanese light pickes, 39 (44.3%) western-style sweets, and 74 (75.5%) Japanese seafood products. Additionally, enterotoxin-producing Staphylococcus aureus strains were isolated from 2 Japanese light pickes, 2 western-style sweets, and 1 Japanese seafood product. On the other hand, the prevalence of C. braakii/freundii, E. cloacae complex, E. coli, K. oxytoca, K. pneumonia, P. mirabilis, and S. marcescens in these RTE foods was determined by bacterial species identifications of Enterobacteriaceae. Moreover, the third-generation cephalosporin-, FOM-, or quinolone-resistant strains were detected by antimicrobial disk susceptibility tests.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (3 results)

All 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Multiplex Real-Time PCR Assays for Screening of Shiga Toxin 1 and 2 Genes, Including All Known Subtypes, and Escherichia coli O26-, O111-, and O157-Specific Genes in Beef and Sprout Enrichment Cultures2015

    • Author(s)
      T. Harada, A. Iguchi, S. Iyoda, K. Seto, M. Taguchi, Y. Kumeda
    • Journal Title

      Journal of Food Protection

      Volume: 78 Issue: 10 Pages: 1800-1811

    • DOI

      10.4315/0362-028x.jfp-15-050

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 浅漬における腸内細菌科菌群汚染実態と分離株の薬剤感受性2015

    • Author(s)
      原田哲也、神吉政史、田口真澄、久米田裕子
    • Organizer
      第36回日本食品微生物学会学術総会
    • Place of Presentation
      川崎市教育文化会館
    • Year and Date
      2015-11-12
    • Related Report
      2015 Annual Research Report
  • [Presentation] ベトナムで流通する香辛料の腸内細菌科菌群汚染実態と分離株の薬剤耐性2015

    • Author(s)
      山根諒子、原田哲也、井澤恭子、河原隆二、久米田裕子、伊勢川裕二、山本容正
    • Organizer
      第36回日本食品微生物学会学術総会
    • Place of Presentation
      川崎市教育文化会館
    • Year and Date
      2015-11-12
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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