Construction of a microfluidic system for the analysis of human blood-placental barrier
| Project/Area Number |
25750157
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Kyoto University (2014)
The University of Tokyo (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 胎盤バリア / 流体シェアストレス / 微絨毛 / カルシウムイオン / TRP channel / TRPV6 / 流体シェア / シェアストレス / グルコーストランスポーター / マイクロ流路 |
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| Outline of Final Research Achievements |
We constructed a microfluidic system that enabled us to analyze the function of human placental barrier under the fluid flow microenvironment. We used a human trophoblastic cell line, human umbilical vein endothelial cells and a vitrified collagen scaffold to reconstruct the polarized multi-layered barrier structure between two microfluidic channels. Using our device, we found that fluid flow induced the formation of microvilli in the placental barrier construct, as observed in the placental barrier tissue, whereas static culture failed to induce microvilli. Fluid shear stress also affected the material transfer through the barrier constructs. We further revealed that the microvilli are induced via a mechanosensitive activation of TRP channel.
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Report
(3 results)
Research Products
(2 results)
