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Construction of a microfluidic system for the analysis of human blood-placental barrier

Research Project

Project/Area Number 25750157
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionKyoto University (2014)
The University of Tokyo (2013)

Principal Investigator

SHIGENORI MIURA  京都大学, 再生医科学研究所, 助教 (70511244)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords胎盤バリア / 流体シェアストレス / 微絨毛 / カルシウムイオン / TRP channel / TRPV6 / 流体シェア / シェアストレス / グルコーストランスポーター / マイクロ流路
Outline of Final Research Achievements

We constructed a microfluidic system that enabled us to analyze the function of human placental barrier under the fluid flow microenvironment. We used a human trophoblastic cell line, human umbilical vein endothelial cells and a vitrified collagen scaffold to reconstruct the polarized multi-layered barrier structure between two microfluidic channels. Using our device, we found that fluid flow induced the formation of microvilli in the placental barrier construct, as observed in the placental barrier tissue, whereas static culture failed to induce microvilli. Fluid shear stress also affected the material transfer through the barrier constructs. We further revealed that the microvilli are induced via a mechanosensitive activation of TRP channel.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2014

All Presentation (2 results)

  • [Presentation] Reconstitution of the human placental barrier on a chip2014

    • Author(s)
      S. Miura, T. Teshima, S. Takeuchi
    • Organizer
      JST ERATO International Symposium on 3D Tissue Fabrication
    • Place of Presentation
      Tokyo
    • Year and Date
      2014-05-20 – 2014-05-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] マイクロ流路デバイスを利用した血液-胎盤関門のin vitro再構築2014

    • Author(s)
      三浦重徳、手島哲彦、竹内昌治
    • Organizer
      日本再生医療学会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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