| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Fluorine-19 magnetic resonance imaging (19F MRI) could be a promising approach for imaging amyloid deposition in the brain. However, the required features of a 19F MRI probe for amyloid detection remain unclear. In the present study, we investigated a series of compounds as potent 19F probes that could prevent the reduction in MR signal when bound to amyloid plaques in the brain. We found that 6-(3’,6’,9’,15’,18’,21’-heptaoxa-23’,23’,23’-trifluorotricosanyloxy)-2-(4’-dimethylaminostyryl)benzoxazole (Shiga-X22), which has a trifluoroethoxy group with seven ethylene glycol groups in the PEG chain, showed significant 19F MR signals in the brains of APPswe/PS1dE9 double-transgenic mice, but not wild-type mice. This suggested that Shiga-X22 could be a useful 19F MRI probe for amyloid detection. Furthermore, we synthesized new probes for neurofibrillary tangles, which has a trifluoroethoxy group with different number of ethylene glycol groups in the PEG chain.
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