| Project/Area Number |
25750206
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Rehabilitation science/Welfare engineering
|
|---|
| Research Institution | Yamaguchi University |
|---|
Principal Investigator |
KIDA Hiroyuki 山口大学, 大学院医学系研究科, 助教 (70432739)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | シナプス可塑性 / 運動学習 / 一次運動野 / AMPA受容体 / グルタミン酸 |
|---|
| Outline of Final Research Achievements |
Synaptic plasticity mediated by AMPA glutamate receptors is highly associated with many types of learning. To analyze plasticity in layers II/III area in primary motor cortex, we subjected rats to a rotor rod test. Motor skill consistently improved within 2 days of training, and we prepared acute brain slices for patch clamp analyses.1-day trained rats exhibited significantly higher AMPA/NMDA ratios and miniature EPSC amplitude than untrained rats, suggesting an increase in postsynaptic AMPA receptors in the early phase of motor learning. Animals trained for 2 days showed a significantly higher miniature EPSC amplitude and frequency than untrained rats. Paired-pulse analysis of evoked EPSC significantly decreased after 2-days training, suggesting increased presynaptic glutamate release at the late phase of learning. Here we revealed that motor training induces dynamic changes in glutamatergic plasticity in layers II/III neurons of the primary motor cortex.
|
