Usefulness of noninvasive monitoring method for tissue-specific energy metabolism in human
| Project/Area Number |
25750334
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Sports science
|
|---|
| Research Institution | Tokyo Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
|---|
| Keywords | 骨格筋 / エネルギー代謝 / 運動 / アミノ酸 / 脂肪 / 脂肪酸 |
|---|
| Outline of Final Research Achievements |
This study examined noninvasive metabolic markers in serum, urine, and saliva by LC-MS/MS system to evaluate energy productive balance among nutrients during and after endurance exercise in human. Immediately after marathon race, high sensitive method could measure the elevations of lactate, acetyl-carnitine as end-production of fatty acid beta-oxidation, 3-hydroxybutyrate (3HB) as ketone body, 3-hydoroxyisobutyrate (3HIB) and 3-hydroxy-3-methylbutyrate as intermediates of valine and leucine catabolism, and N-acetyltaurine (NAT) as a metabolite of acetate in serum. After a transient endurance running, urine NAT excretion was also observed over time. In addition, this method could observe the real-time energy metabolism of lipid and amino acid during and after the endurance exercise by measuring of 3HB and 3HIB in saliva. The results confirmed that the high-sensitive detective method was useful for noninvasive and real-time monitoring of tissue-specific energy metabolism in human.
|
Report
(4 results)
Research Products
(25 results)
