| Project/Area Number |
25750389
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomolecular chemistry
|
|---|
| Research Institution | Tokyo University of Science |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 活性発現の分子機構 / 標的タンパク質 / 細胞分裂 / 紡錘体 / アフィニティーラベル |
|---|
| Outline of Final Research Achievements |
Pyrenocine A induces monopolar spindle against HeLa cells. To reveal the mechanism of action, we analyzed the localization of pyrenocine A in a cell using pyrenocine A-alkyne derivative. As a result, the derivative was found to localize to centrosome, which suggested that pyrenocine A bound to proteins at centrosome. To identify target proteins of pyrenocine A, we purified pyrenocine A-binding proteins using pyrenocine A-alkyne derivatives. The obtained binding proteins were found to be enolase 1 and elongation factor 1-alpha 1 by LCMS/MS analysis.
|
