| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Genetic engineering technologies for regulating intracellular target-gene function and/or expression have been widely used for basic research of vital biological phenomena. RNA mutagenesis has the potential to become a powerful gene-regulation methodology as an alternative to DNA mutagenesis. Adenosine-to-inosine (A-to-I) RNA editing that alters genetic information at the transcript level is an important biological process widely conserved in metazoans. Therefore, a versatile RNA mutagenesis method can be developed by taking advantage of intracellular the RNA editing mechanism. Here, we developed a novel guide RNA that is capable of inducing A-to-I mutations by guiding the editing enzyme, human adenosine deaminase acting on RNA.
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