Construction of a guide-RNA for site-directed RNA mutagenesis utilizing intracellular A-to-I RNA editing
| Project/Area Number |
25750390
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomolecular chemistry
|
|---|
| Research Institution | Fukuoka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | RNA編集 / ガイドRNA / RNA変異導入 / in vitroセレクション / 分子設計 / 機能性RNA / ADAR / 部位特異的変異導入 |
|---|
| Outline of Final Research Achievements |
Genetic engineering technologies for regulating intracellular target-gene function and/or expression have been widely used for basic research of vital biological phenomena. RNA mutagenesis has the potential to become a powerful gene-regulation methodology as an alternative to DNA mutagenesis. Adenosine-to-inosine (A-to-I) RNA editing that alters genetic information at the transcript level is an important biological process widely conserved in metazoans. Therefore, a versatile RNA mutagenesis method can be developed by taking advantage of intracellular the RNA editing mechanism. Here, we developed a novel guide RNA that is capable of inducing A-to-I mutations by guiding the editing enzyme, human adenosine deaminase acting on RNA.
|
Report
(4 results)
Research Products
(26 results)
