Research Project
Grant-in-Aid for Young Scientists (B)
This study aimed to modulate the cleavage of pre-miRNAs (miRNA precursors) by a small-molecule compound. Small-molecule compounds that bind to a specific sequence in the pre-miRNA have been used to alter the cleavage reaction with Dicer, and thereby increase/decrease the production of the miRNA. Terminal loop sequences of pre-miRNA that bind to our newly designed naphthyridine derivative RND were obtained by in vitro selection from a pre-miR-29a mutant library with the randomized terminal loop region. Binding of RND to the obtained terminal sequences significantly interfere with the cleavage of the terminal loop mutants of pre-miR-29a. Gel shift analysis suggested that the inhibitory effect of RND might be caused by the formation of loop-loop complex between two terminal loop mutants upon binding of RND to the terminal loop.
All 2015 2014 2013
All Journal Article (4 results) (of which Peer Reviewed: 4 results, Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 4 results, Invited: 1 results)
Chem. Eur. J.
Volume: 21 Issue: 47 Pages: 16859-16867
10.1002/chem.201502913
Bull. Chem. Soc. Jpn.
Volume: 87 Issue: 9 Pages: 1013-1015
10.1246/bcsj.20140137
130004687252
Bioorg. Med. Chem. Lett.
Volume: 23 Issue: 12 Pages: 3539-3541
10.1016/j.bmcl.2013.04.037
Bioorg. Med. Chem.
Volume: 21 Issue: 22 Pages: 7101-7106
10.1016/j.bmc.2013.09.007